Polyamine biosynthesis during somatic embryogenesis in interior spruce (Picea glauca x Picea engelmannii complex).

Abstract:

:Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embryos. Polyclonal antibodies raised against Escherichia coli arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), following affinity purification specifically recognized spruce ADC and ODC, which corresponded to 85kD and 65kD bands on western blots of total protein extracts from embryogenic masses, Immunoassays using these antibodies showed increased ADC levels corresponding to embryo maturation while ODC levels remained the same. From these results it is concluded that polyamines are involved in the maturation of somatic embryos of interior spruce.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Amarasinghe V,Dhami R,Carlson JE

doi

10.1007/BF00232981

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

495-9

issue

7

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

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