Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

Abstract:

:Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while A281 produced the heaviest tumors in vitro. Tumor formation and opine production are indicative of plant cell transformation and tumors produced appropriate opines: nopaline (C58), octopine (Ach5 and GV3111), and agropine and mannopine (A281). Southern analysis of DNA from a tumor line produced by strain C58 showed that a T-DNA fragment had been transferred into the lentil genome.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Warkentin TD,McHughen A

doi

10.1007/BF00234579

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

489-93

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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