Abstract：
:A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed by culture in induction medium supplemented with 21.48 muM naphthalene acetic acid and 2.22 muM benzyladenine for 8 weeks, and successive transfer of explants to expression media with a low concentration of growth regulators and without them. Both types of explants formed callus tissue from which somatic embryos developed, indicating indirect embryogenesis. Although the embryogenic frequencies were lower than 12%, it did not prevent the establishment of clonal embryogenic lines maintained by repetitive embryogenesis. Histological study confirmed an indirect somatic embryogenesis process from shoot tip explants, in which leaf primordia and the corresponding axial zones were involved in generating callus, whereas the apical meristem itself did not proliferate. The origin of embryogenic cells appeared to be associated with dedifferentiation of certain parenchymal cells in callus regions after transfer of explants to expression media without auxin. Division of embryogenic cells gave rise to proembryo aggregates of unicellular origin, although a multicellular origin from bulging embryogenic areas would also seem possible. Further development led to the formation of cotyledonary-stage somatic embryos and nodular embryogenic structures that may be considered as anomalous embryos with no clear bipolarity. Inducement of somatic embryos from explants isolated from shoot cultures ensures plant material all year round, thus providing a significant advantage over the use of leaf explants from field-grown trees.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AMdoi
10.1007/s00299-010-0852-6subject
Has Abstractpub_date
2010-06-01 00:00:00pages
661-71issue
6eissn
0721-7714issn
1432-203Xjournal_volume
29pub_type
杂志文章abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0659-9
更新日期：2003-08-01 00:00:00
abstract：:Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232783
更新日期：1995-05-01 00:00:00
abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232042
更新日期：1991-06-01 00:00:00
abstract：:Cell suspension cultures of chickpea (Cicer arietinum L.) were established from cultivars ILC 3279 and ILC 1929, resistant and susceptible towards the chickpea pathogenic fungus Ascochyta rabiei. The two cell culture lines possess identical growth properties and show high accumulation of the isoflavones biochanin A an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269739
更新日期：1987-02-01 00:00:00
abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s002990100346
更新日期：2001-07-01 00:00:00
abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0255-x
更新日期：2007-04-01 00:00:00
abstract：:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232038
更新日期：1995-02-01 00:00:00
abstract：:Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0594-x
更新日期：2008-11-01 00:00:00
abstract：:In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0988-4
更新日期：2011-05-01 00:00:00
abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1911-9
更新日期：2016-03-01 00:00:00
abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0230-6
更新日期：2007-03-01 00:00:00
abstract：:Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050294
更新日期：1997-06-01 00:00:00
abstract：:Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232374
更新日期：1996-01-01 00:00:00
abstract：:An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890875
更新日期：1996-12-01 00:00:00
abstract：:Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050556
更新日期：1998-12-01 00:00:00
abstract：KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02436-8
更新日期：2019-09-01 00:00:00
abstract：:Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269565
更新日期：1987-10-01 00:00:00
abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235072
更新日期：1992-06-01 00:00:00
abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1832-7
更新日期：2015-10-01 00:00:00
abstract：:In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain A(w) in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binar...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1045-7
更新日期：2011-07-01 00:00:00
abstract：:Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF02441015
更新日期：1984-06-01 00:00:00
abstract：:Histone deacetylases (HDACs) mediate histone deacetylation and act in concert with histone acetyltransferases to regulate dynamic and reversible histone acetylation which modifies chromatin structure and function, affects gene transcription, thus, controlling multiple cellular processes. HDACs are widely distributed i...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-013-1393-6
更新日期：2013-04-01 00:00:00
abstract：:Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272634
更新日期：1982-12-01 00:00:00
abstract：: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000220
更新日期：2000-10-01 00:00:00
abstract：:Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1118-7
更新日期：2011-11-01 00:00:00
abstract：:Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0226-2
更新日期：2007-03-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1725-1
更新日期：2015-03-01 00:00:00
abstract：:The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1028-8
更新日期：2011-07-01 00:00:00
abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0836-5
更新日期：2004-10-01 00:00:00
