Knockdown of NtMed8, a Med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (Nicotiana tabacum L.).

Abstract:

:Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still unknown. To investigate the function of Med8 ortholog in tobacco (Nicotiana tabacum L.; named as NtMed8), we created transgenic tobacco plants with repressed NtMed8 expression mediated by RNA interference (RNAi). Compared with the wild type, the NtMed8-RNAi plants exhibited: more leaves with smaller but thicker blades; larger cells and vascular bundles with lower stomata density in leaves; swelled chloroplasts with thicker and lumen-enlarged thylakoids; weaker root system with fewer lateral roots; larger flowers and floral organs; flowering earlier under long day, but later under short day conditions; and male sterile with larger but less germinable pollens. In addition, quantitative RT-PCR indicated that NtMed8 is expressed in both vegetative and floral tissues. Subcellular localization analysis by transient expression of fusion protein in Nicotiana benthamiana leaves showed that NtMed8 was located in both plasma membrane and nucleus. These results suggest that NtMed8 plays important roles in both vegetative and reproductive development, and the function of Med8 appears to be, at least partially, conserved in flowering plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Wang F,Wei H,Tong Z,Zhang X,Yang Z,Lan T,Duan Y,Wu W

doi

10.1007/s00299-011-1118-7

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

2117-29

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

30

pub_type

杂志文章
  • Gravity and light control of the developmental polarity of regenerating protoplasts isolated from prothallial cells of the fern Ceratopteris richardii.

    abstract::A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050470

    authors: Edwards ES,Roux SJ

    更新日期:1998-06-01 00:00:00

  • Tissue culture of Chrysosplenium americanum and its potential for flavonoid production.

    abstract::Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270122

    authors: Brisson L,Ibrahim RK,Rideau M

    更新日期:1988-03-01 00:00:00

  • Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees.

    abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0604-y

    authors: Hernández I,Celestino C,Alegre J,Toribio M

    更新日期:2003-04-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232275

    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Polyamines and crown gall tumor growth.

    abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269212

    authors: Kulpa JM,Galsky AG,Lipetz P,Stephens R

    更新日期:1985-04-01 00:00:00

  • In vitro pollination of maize (Zea mays L.) - Proof of double fertilization.

    abstract::Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00267652

    authors: Havel L,Novák FJ

    更新日期:1981-08-01 00:00:00

  • Improving the efficiency of isolated microspore culture in six-row spring barley: I-optimization of key physical factors.

    abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1583-x

    authors: Esteves P,Belzile F

    更新日期:2014-06-01 00:00:00

  • Non-antibiotic, efficient selection for alfalfa genetic engineering.

    abstract::A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0321-z

    authors: Rosellini D,Capomaccio S,Ferradini N,Savo Sardaro ML,Nicolia A,Veronesi F

    更新日期:2007-07-01 00:00:00

  • Plant cell cultures as food-aspects of sustainability and safety.

    abstract:KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02592-2

    authors: Häkkinen ST,Nygren H,Nohynek L,Puupponen-Pimiä R,Heiniö RL,Maiorova N,Rischer H,Ritala A

    更新日期:2020-12-01 00:00:00

  • Reduced glutathione promotes callus growth and shoot development in a shoot tip culture of apple root stock M26.

    abstract::Reduced glutathione (GSH) was applied to prevent browning in shoot tip explants of apple (Malus pumila Mill.). Development was compared between shoot tips treated either by (a) dipping into 0.1 mM GSH solution prior to culture (dip treatment), (b) dipping into the GSH solution and transferring to a medium containing 0...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050449

    authors: Nomura K,Matsumoto S,Masuda K,Inoue M

    更新日期:1998-05-01 00:00:00

  • Isolation and viral infection of Capsicum leaf protoplasts.

    abstract::A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234146

    authors: Murphy JF,Kyle MM

    更新日期:1994-04-01 00:00:00

  • Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America.

    abstract::Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272536

    authors: Davis DG,Olson PA,Stolzenberg RL

    更新日期:1988-06-01 00:00:00

  • Effect of 1-Aminocyclopropane-1-carboxylic acid and aminoethoxyvinylglycine on ethylene emanation and somatic embryogenesis from orchardgrass leaf cultures.

    abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272059

    authors: Songstad DD,Petracek PD,Sams CE,Conger BV

    更新日期:1989-03-01 00:00:00

  • In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.

    abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0230-6

    authors: Guo B,Gao M,Liu CZ

    更新日期:2007-03-01 00:00:00

  • Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum.

    abstract::Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and B...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00236268

    authors: Angeloni PN,Rey HY,Mroginski LA

    更新日期:1992-09-01 00:00:00

  • Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf.

    abstract::The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0095-0

    authors: Kim MK,Lee BS,In JG,Sun H,Yoon JH,Yang DC

    更新日期:2006-06-01 00:00:00

  • Auxin and gibberellin responsive Arabidopsis SMALL AUXIN UP RNA36 regulates hypocotyl elongation in the light.

    abstract:KEY MESSAGE:The Arabidopsis SAUR36, renamed RAG1, integrates auxin and gibberellin signals to regulate apical hook maintenance in etiolated seedlings, hypocotyl elongation in the light and fertility. Phytohormone signalling intermediates integrate responses to developmental cues and the variety of environmental inputs ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-013-1406-5

    authors: Stamm P,Kumar PP

    更新日期:2013-06-01 00:00:00

  • Rapid micropropagation for Mucuna pruriens f. pruriens L.

    abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193734

    authors: Chattopadhyay S,Datta SK,Mahato SB

    更新日期:1995-12-01 00:00:00

  • Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

    abstract::Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234579

    authors: Warkentin TD,McHughen A

    更新日期:1991-12-01 00:00:00

  • TaUBA, a UBA domain-containing protein in wheat (Triticum aestivum L.), is a negative regulator of salt and drought stress response in transgenic Arabidopsis.

    abstract:KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1739-3

    authors: Li X,Zhang SS,Ma JX,Guo GY,Zhang XY,Liu X,Bi CL

    更新日期:2015-05-01 00:00:00

  • Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones.

    abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1183-y

    authors: Prabu G,Prasad DT

    更新日期:2012-04-01 00:00:00

  • Effect of yeast elicitor on the secondary metabolism of Ti-transformed Salvia miltiorrhiza cell suspension cultures.

    abstract:: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. milti...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002999900166

    authors: Chen H,Chen F

    更新日期:2000-06-01 00:00:00

  • 5'-Anchored simple-sequence repeat primers are useful for analysing potato somatic hybrids.

    abstract::Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050735

    authors: Matthews D,McNicoll J,Harding K,Millam S

    更新日期:1999-12-01 00:00:00

  • Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.).

    abstract:KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1670-z

    authors: Wan F,Pan Y,Li J,Chen X,Pan Y,Wang Y,Tian S,Zhang X

    更新日期:2014-12-01 00:00:00

  • Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid.

    abstract::Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232412

    authors: Kurioka Y,Suzuki Y,Kamada H,Harada H

    更新日期:1992-12-01 00:00:00

  • Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja.

    abstract::The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716844

    authors: Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

    更新日期:1989-02-01 00:00:00

  • Functional characterization of a chalcone synthase from the liverwort Plagiochasma appendiculatum.

    abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1702-8

    authors: Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

    更新日期:2015-02-01 00:00:00

  • Propagation of fern (Nephrolepis) through tissue culture.

    abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272634

    authors: Padhya MA,Mehta AR

    更新日期:1982-12-01 00:00:00

  • Clonal propagation of Picrorhiza kurroa royle ex benth. by shoot tip culture.

    abstract::A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269324

    authors: Lal N,Ahuja PS,Kukreja AK,Pandey B

    更新日期:1988-05-01 00:00:00

  • Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.).

    abstract::Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269240

    authors: Yamada Y,Yang ZQ,Tang DT

    更新日期:1986-04-01 00:00:00