Functional characterization of a chalcone synthase from the liverwort Plagiochasma appendiculatum.

Abstract:

KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It produces naringenin chalcone by condensing one p-coumaroyl- and three malonyl-coenzyme A thioesters through a polyketide intermediate that is cyclized by intramolecular Claisen condensation. Although CHSs of higher plants have been extensively studied, enzyme properties of the CHSs in liverworts have been scarcely characterized. In this study, we report the cloning and characterization of CHS (designated as PaCHS) from the liverwort Plagiochasma appendiculatum. The gene product was 60-70 % identical with chalcone synthases from other species, and contained the characteristic conserved Cys-His-Asn catalytic triad. The recombinant PaCHS was able to catalyze p-coumaroyl-CoA and malonyl-CoA to generate naringenin in vitro. Heterologously expressed PaCHS protein showed similar kinetic properties to those of higher plant CHS. The ectopic expression of PaCHS in Marchantia paleacea callus raised the content of the total flavonoids. These results suggested that PaCHS played a key role in the flavonoids biosynthesis in liverworts. Furthermore, when the thallus of P. appendiculatum was treated with abiotic stress inducers methyl jasmonate, salicylic acid and abscisic acid, PaCHS expression was enhanced. This is the first time that a CHS in liverworts has been functionally characterized.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

doi

10.1007/s00299-014-1702-8

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

233-45

issue

2

eissn

0721-7714

issn

1432-203X

journal_volume

34

pub_type

杂志文章
  • Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration.

    abstract::We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050311

    authors: Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

    更新日期:1997-09-01 00:00:00

  • Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants.

    abstract:: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990000220

    authors: Nobre J,Davey MR,Lazzeri PA,Cannell ME

    更新日期:2000-10-01 00:00:00

  • Overexpression of WssgtL3.1 gene from Withania somnifera confers salt stress tolerance in Arabidopsis.

    abstract:KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-021-02666-9

    authors: Mishra MK,Tiwari S,Misra P

    更新日期:2021-02-01 00:00:00

  • Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture.

    abstract::The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0534-9

    authors: Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Swierczyńska J,Góralski G,Slesak H,Bohdanowicz J

    更新日期:2008-07-01 00:00:00

  • Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

    abstract::Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269528

    authors: Yang H,Zhang HM,Davey MR,Mulligan BJ,Cocking EC

    更新日期:1988-10-01 00:00:00

  • The cation-efflux transporter BjCET2 mediates zinc and cadmium accumulation in Brassica juncea L. leaves.

    abstract::Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-009-0723-1

    authors: Xu J,Chai T,Zhang Y,Lang M,Han L

    更新日期:2009-08-01 00:00:00

  • Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures.

    abstract::A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232422

    authors: Su RC,Rudert ML,Hodges TK

    更新日期:1992-12-01 00:00:00

  • Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis.

    abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2068-x

    authors: Teng K,Tan P,Xiao G,Han L,Chang Z,Chao Y

    更新日期:2017-01-01 00:00:00

  • FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana.

    abstract:KEY MESSAGE:Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02634-9

    authors: Ayabe H,Kawai N,Shibamura M,Fukao Y,Fujimoto M,Tsutsumi N,Arimura SI

    更新日期:2021-02-01 00:00:00

  • Role of inhibitors in the induction of differentiation in callus cultures of Brassica, Datura and Nicotiana.

    abstract::The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270062

    authors: Sethi U,Basu A,Mukherjee SG

    更新日期:1990-03-01 00:00:00

  • Complete sequence and organization of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome.

    abstract::The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0097-y

    authors: Kim JS,Jung JD,Lee JA,Park HW,Oh KH,Jeong WJ,Choi DW,Liu JR,Cho KY

    更新日期:2006-04-01 00:00:00

  • Expression analysis of defense-related genes in Zingiber (Zingiberaceae) species with different levels of compatibility to the soft rot pathogen Pythium aphanidermatum.

    abstract::Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0594-x

    authors: Kavitha PG,Thomas G

    更新日期:2008-11-01 00:00:00

  • Immunolocalization of lipoxygenase in pea (Pisum sativum L.) carpels.

    abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232465

    authors: Rodríguez-Concepción M,Gómez MD,Beltrán JP

    更新日期:1996-04-01 00:00:00

  • The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition.

    abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234368

    authors: Toivonen L,Laakso S,Rosenqvist H

    更新日期:1992-07-01 00:00:00

  • Auxin-polyamine interaction with the calcium-controlled secretion of peroxidase by sugarbeet cells.

    abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00571295

    authors: Kevers C,Gaspar T,Penel C,Greppin H

    更新日期:1985-05-01 00:00:00

  • Root-targeted biotechnology to mediate hormonal signalling and improve crop stress tolerance.

    abstract::Since plant root systems capture both water and nutrients essential for the formation of crop yield, there has been renewed biotechnological focus on root system improvement. Although water and nutrient uptake can be facilitated by membrane proteins known as aquaporins and nutrient transporters, respectively, there is...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-011-1005-2

    authors: Ghanem ME,Hichri I,Smigocki AC,Albacete A,Fauconnier ML,Diatloff E,Martinez-Andujar C,Lutts S,Dodd IC,Pérez-Alfocea F

    更新日期:2011-05-01 00:00:00

  • Polyamines and crown gall tumor growth.

    abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269212

    authors: Kulpa JM,Galsky AG,Lipetz P,Stephens R

    更新日期:1985-04-01 00:00:00

  • The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.

    abstract::The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0917-0

    authors: Shirasawa-Seo N,Sano Y,Nakamura S,Murakami T,Gotoh Y,Naito Y,Hsia CN,Seo S,Mitsuhara I,Kosugi S,Ohashi Y

    更新日期:2005-06-01 00:00:00

  • Fruit quality and DNA methylation are affected by parental order in reciprocal crosses of tomato.

    abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02624-x

    authors: Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

    更新日期:2021-01-01 00:00:00

  • Differential expression of gibberellin- and abscisic acid-related genes implies their roles in the bud activity-dormancy transition of tea plants.

    abstract:KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2238-5

    authors: Yue C,Cao H,Hao X,Zeng J,Qian W,Guo Y,Ye N,Yang Y,Wang X

    更新日期:2018-03-01 00:00:00

  • Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.).

    abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050481

    authors: Ayabe M,Sumi S

    更新日期:1998-07-01 00:00:00

  • Effect of gibberellin biosynthesis inhibitors on shoot regeneration from hypocotyl explants of Albizzia julibrissin.

    abstract::Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00235303

    authors: Sankhla D,Davis TD,Sankhla N

    更新日期:1993-12-01 00:00:00

  • The role of Ca(2+) in elicitation of phytoalexin synthesis in cell culture of onion (Allium cepa L.).

    abstract::Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231593

    authors: Dmitriev A,Djatsok J,Grodzinsky D

    更新日期:1996-09-01 00:00:00

  • Abscisic acid-regulated Glb1 transient expression in cultured maize P3377 cells.

    abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050459

    authors: Liu S,Kriz A,Duncan D,Widholm J

    更新日期:1998-05-01 00:00:00

  • Rapid micropropagation for Mucuna pruriens f. pruriens L.

    abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193734

    authors: Chattopadhyay S,Datta SK,Mahato SB

    更新日期:1995-12-01 00:00:00

  • Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees.

    abstract::A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0852-6

    authors: San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AM

    更新日期:2010-06-01 00:00:00

  • Inhibition of somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.] by butylated hydroxytoluene, a volatile antioxidant released by parafilm.

    abstract::Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01890865

    authors: Selby C,McRoberts WC,Hamilton JT,Harvey BM

    更新日期:1996-12-01 00:00:00

  • Effect of basal medium, growth regulators and Phytagel concentration on initiation of embryogenic cultures from immature zygotic embryos of loblolly pine (Pinus taeda L.).

    abstract::Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050396

    authors: Li XY,Huang FH,Gbur EE Jr

    更新日期:1998-02-01 00:00:00

  • High plating efficiency with plant cell cultures.

    abstract::In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony tra...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00571305

    authors: Bellincampi D,Baduri N,Morpurgo G

    更新日期:1985-05-01 00:00:00

  • A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation.

    abstract:KEY MESSAGE:SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieve...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2211-3

    authors: Guo JE,Hu Z,Yu X,Li A,Li F,Wang Y,Tian S,Chen G

    更新日期:2018-01-01 00:00:00