Inhibition of somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.] by butylated hydroxytoluene, a volatile antioxidant released by parafilm.

abstract：:In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 year...

journal_title：Plant cell reports

authors： Smýkal P,Valledor L,Rodríguez R,Griga M

更新日期：2007-11-01 00:00:00

abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...

journal_title：Plant cell reports

authors： Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers L

更新日期：2003-11-01 00:00:00

abstract：:Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...

journal_title：Plant cell reports

authors： Kim TG,Baek MY,Lee EK,Kwon TH,Yang MS

更新日期：2008-05-01 00:00:00

abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...

journal_title：Plant cell reports

authors： Kang L,Du X,Zhou Y,Zhu B,Ge X,Li Z

更新日期：2014-08-01 00:00:00

abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

journal_title：Plant cell reports

authors： Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

更新日期：2011-12-01 00:00:00

abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...

journal_title：Plant cell reports

authors： Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

更新日期：2000-11-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...

journal_title：Plant cell reports

authors： Mishra MK,Tiwari S,Misra P

更新日期：2021-02-01 00:00:00

abstract：:Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expressi...

journal_title：Plant cell reports

authors： Conrath U,Klessig DF,Bachmair A

更新日期：1998-08-01 00:00:00

abstract：:We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that A...

journal_title：Plant cell reports

authors： van Roekel JS,Damm B,Melchers LS,Hoekema A

更新日期：1993-09-01 00:00:00

abstract：KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultiva...

journal_title：Plant cell reports

authors： Anbazhagan K,Bhatnagar-Mathur P,Vadez V,Dumbala SR,Kishor PB,Sharma KK

更新日期：2015-02-01 00:00:00

abstract：:We report here the successful establishment of callus, cell and root cultures from explants of in-vitro-grown plantlets of the soapwort Saponaria officinalis L. The production of saporin in the different tissue systems was evaluated by determining the capability of crude extracts to inactivate protein synthesis and by...

journal_title：Plant cell reports

authors： Di Cola A,Di Domenico C,Poma A,Spanò L

更新日期：1997-11-01 00:00:00

abstract：:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...

journal_title：Plant cell reports

authors： Igasaki T,Sato T,Akashi N,Mohri T,Maruyama E,Kinoshita I,Walter C,Shinohara K

更新日期：2003-11-01 00:00:00

abstract：:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

journal_title：Plant cell reports

authors： Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

更新日期：2007-11-01 00:00:00

abstract：:A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

journal_title：Plant cell reports

authors： Su RC,Rudert ML,Hodges TK

更新日期：1992-12-01 00:00:00

abstract：:The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...

journal_title：Plant cell reports

authors： Polisetty R,Paul V,Deveshwar JJ,Khetarpal S,Suresh K,Chandra R

更新日期：1997-05-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

journal_title：Plant cell reports

authors： Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

更新日期：1989-02-01 00:00:00

abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...

journal_title：Plant cell reports

authors： Hall RD,Pedersen C,Krens FA

更新日期：1993-04-01 00:00:00

abstract：:The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

journal_title：Plant cell reports

authors： Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

更新日期：2011-04-01 00:00:00

abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...

journal_title：Plant cell reports

authors： Ayyappan V,Saha MC,Thimmapuram J,Sripathi VR,Bhide KP,Fiedler E,Hayford RK,Kalavacharla VK

更新日期：2017-01-01 00:00:00

abstract：:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...

journal_title：Plant cell reports

authors： Ueno K,Fukunaga Y,Arisumi K

更新日期：1996-11-01 00:00:00

abstract：:Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...

journal_title：Plant cell reports

authors： Tang W

更新日期：2003-02-01 00:00:00

abstract：:Capsella bursa-pastoris (L.) Medic (2n = 4x = 32) is a natural double-low (erucic acid < 1%, glucosinolates < 30 micromol/g) germplasm and shows high degree of resistance to Sclerotinia sclerotiorum. Hybridizations were carried out between two Brassica species viz. B. rapa (2n = 20) and B. napus (2n = 38) as female an...

journal_title：Plant cell reports

authors： Chen HF,Wang H,Li ZY

更新日期：2007-10-01 00:00:00

abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

journal_title：Plant cell reports

authors： Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

更新日期：2012-06-01 00:00:00

abstract：:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...

journal_title：Plant cell reports

authors： Hagio T,Hirabayashi T,Machii H,Tomotsune H

更新日期：1995-02-01 00:00:00

abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...

journal_title：Plant cell reports

authors： Kuchuk N,Komarnitski I,Shakhovsky A,Gleba Y

更新日期：1990-04-01 00:00:00

abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

journal_title：Plant cell reports

authors： Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

更新日期：2007-03-01 00:00:00

abstract：:In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...

journal_title：Plant cell reports

authors： Fermin G,Tennant P

更新日期：2011-05-01 00:00:00

abstract：KEY MESSAGE:Transcription factors from mammals and plants, which play a role in innate immunity, interact with the same microbe-associated molecular pattern (MAMP)-responsive sequences from Arabidopsis thaliana. The interaction of mouse NF-κB p65 with MAMP-responsive sequences containing the core motif GACTTT of the WT...

journal_title：Plant cell reports

authors： Kanofsky K,Riggers J,Staar M,Strauch CJ,Arndt LC,Hehl R

更新日期：2019-09-01 00:00:00