Abstract：
KEY MESSAGE:Transcription factors from mammals and plants, which play a role in innate immunity, interact with the same microbe-associated molecular pattern (MAMP)-responsive sequences from Arabidopsis thaliana. The interaction of mouse NF-κB p65 with MAMP-responsive sequences containing the core motif GACTTT of the WT-box was investigated. This revealed one sequence, derived from the promoter of the A. thaliana gene At1g76960, a gene with unknown function, to activate NF-κB p65 dependent reporter gene expression in plant cells very strongly. A bioinformatic analysis predicts three putative NF-κB p65 binding sites in this sequence and all three sites are required for reporter gene activation and binding. The sequence is one of the weakest MAMP-responsive sequences previously isolated, but the introduction of a GCC-box increases its MAMP responsivity in combination with upstream WT-box sequences. Although a bioinformatic analysis of the unmutated cis-sequence only predicts NF-κB p65 binding, A. thaliana WRKY40 was selected in a yeast one-hybrid screen. WRKY40, which is a transcriptional repressor, requires the sequence TTTTCTA for direct binding. This sequence is similar to the WK-box TTTTCCAC, previously shown to interact with tobacco NtWRKY12. In summary, this work supports the similarity in binding site recognition between NF-κB and WRKY factors.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kanofsky K,Riggers J,Staar M,Strauch CJ,Arndt LC,Hehl Rdoi
10.1007/s00299-019-02433-xsubject
Has Abstractpub_date
2019-09-01 00:00:00pages
1139-1150issue
9eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-019-02433-xjournal_volume
38pub_type
杂志文章abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0250-2
更新日期：2007-04-01 00:00:00
abstract：:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232038
更新日期：1995-02-01 00:00:00
abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01690249
更新日期：1995-01-01 00:00:00
abstract：:Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0672-z
更新日期：2003-09-01 00:00:00
abstract：KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1667-7
更新日期：2014-11-01 00:00:00
abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00778536
更新日期：1989-04-01 00:00:00
abstract：:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231809
更新日期：1991-03-01 00:00:00
abstract：:High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01092774
更新日期：1997-04-01 00:00:00
abstract：:Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0176-8
更新日期：2006-11-01 00:00:00
abstract：:Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0848-1
更新日期：2004-12-01 00:00:00
abstract：:A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1170-3
更新日期：2012-02-01 00:00:00
abstract：:Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0863-3
更新日期：2010-07-01 00:00:00
abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0937-9
更新日期：2005-08-01 00:00:00
abstract：: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000234
更新日期：2000-11-01 00:00:00
abstract：:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0412-x
更新日期：2007-11-01 00:00:00
abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232295
更新日期：1990-09-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268477
更新日期：1987-06-01 00:00:00
abstract：:The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-009-0741-z
更新日期：2009-09-01 00:00:00
abstract：:To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236381
更新日期：1992-11-01 00:00:00
abstract：:Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232981
更新日期：1996-03-01 00:00:00
abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272780
更新日期：1987-12-01 00:00:00
abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193714
更新日期：1995-12-01 00:00:00
abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235258
更新日期：1992-03-01 00:00:00
abstract：:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233096
更新日期：1992-10-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
abstract：:Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231924
更新日期：1996-05-01 00:00:00
abstract：KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1663-y
更新日期：2014-11-01 00:00:00
abstract：: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050776
更新日期：2000-05-01 00:00:00
abstract：:Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-004-0858-z
更新日期：2004-11-01 00:00:00
