A novel elicitor identified from Magnaporthe oryzae triggers defense responses in tobacco and rice.

Abstract:

KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from an important pathogenic fungus, Magnaporthe oryzae. The protein fraction was isolated from the culture filtrate of M. oryzae and identified by de novo sequencing. The elicitor-encoding gene mohrip2 was cloned following sequence comparison and PCR amplification. This 459-bp gene encodes a 152-residue polypeptide that contains an 18-residue signal peptide and exhibits a pI of 4.72 and an apparent molecular mass of 16 kDa. The hypothetical protein, MoHrip2, was expressed in Escherichia coli, and both the recombinant and the endogenous protein caused necrotic lesions in tobacco leaves. In addition to phenolic compound deposition and alkalization of the extracellular medium, MoHrip2 also induced hydrogen peroxide production and nitric oxide accumulation in tobacco cells. Moreover, rice seedlings treated with MoHrip2 exhibited pronounced resistance to M. oryzae compared with control seedlings.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Chen M,Zhang C,Zi Q,Qiu D,Liu W,Zeng H

doi

10.1007/s00299-014-1663-y

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

1865-79

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

33

pub_type

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