Abstract：
:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is the first report of the introduction of T-DNA into P. nil and the T-DNA has been shown to be integrated without apparent rearrangement in its genome. The range of copy numbers was between 3 and 5. The beta-glucuronidase activities measured were about 10 times higher than those of transgenic tobacco by introduction of the same plasmid as previously described by Jefferson et al. (1987). Thus, the widely used CaMV 35S promoter also appears to be very active in P. nil.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Araki T,Hirano H,Naito S,Komeda Ydoi
10.1007/BF00274124subject
Has Abstractpub_date
1989-05-01 00:00:00pages
259-62issue
5eissn
0721-7714issn
1432-203Xjournal_volume
8pub_type
杂志文章abstract：:A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233766
更新日期：1994-12-01 00:00:00
abstract：:Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0621-x
更新日期：2003-08-01 00:00:00
abstract：:We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide ge...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269646
更新日期：1986-12-01 00:00:00
abstract：:Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1320-2
更新日期：2012-11-01 00:00:00
abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
abstract：KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2332-3
更新日期：2018-12-01 00:00:00
abstract：:Three O-methyltransferases which catalyze S-adenosyl-L-methionine (SAM)-dependent O-methylation of licodione (LMT), flavone/flavonol (FMT), and caffeic acid (CMT) were separated from the callus culture of Glycyrrhiza echinata, and characteristic differences between their pH optima and Mg(2+) requirement for activity w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269729
更新日期：1987-02-01 00:00:00
abstract：:Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232330
更新日期：1991-02-01 00:00:00
abstract：:A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0909-6
更新日期：2010-11-01 00:00:00
abstract：:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0350-7
更新日期：2007-08-01 00:00:00
abstract：:Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890865
更新日期：1996-12-01 00:00:00
abstract：KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2039-2
更新日期：2016-11-01 00:00:00
abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269825
更新日期：1986-08-01 00:00:00
abstract：:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232608
更新日期：1991-10-01 00:00:00
abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193714
更新日期：1995-12-01 00:00:00
abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232092
更新日期：1990-07-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1725-1
更新日期：2015-03-01 00:00:00
abstract：:The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0542-0
更新日期：2003-01-01 00:00:00
abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02548-6
更新日期：2020-08-01 00:00:00
abstract：KEY MESSAGE:Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02502-1
更新日期：2020-04-01 00:00:00
abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268477
更新日期：1987-06-01 00:00:00
abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0422-8
更新日期：2008-01-01 00:00:00
abstract：:The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234289
更新日期：1991-07-01 00:00:00
abstract：:Cell suspension cultures of chickpea (Cicer arietinum L.) were established from cultivars ILC 3279 and ILC 1929, resistant and susceptible towards the chickpea pathogenic fungus Ascochyta rabiei. The two cell culture lines possess identical growth properties and show high accumulation of the isoflavones biochanin A an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269739
更新日期：1987-02-01 00:00:00
abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234146
更新日期：1994-04-01 00:00:00
abstract：:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0129-2
更新日期：2006-07-01 00:00:00
abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231832
更新日期：1992-02-01 00:00:00
abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2274-9
更新日期：2018-06-01 00:00:00
abstract：:A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269202
更新日期：1985-04-01 00:00:00
