Abstract：
:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an intensity of 300 muW cm(-2) led to reduced viability, especially under long irradiation duration. The protoplasts could not grow during culture when they were irradiated for over 30 s. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay revealed extensive DNA fragmentation in the UV-irradiated protoplasts compared with those without UV treatment. Electrofusion between UV-irradiated protoplasts of Satsuma mandarin (donor) with those of Jincheng (C. sinensis Osbeck, recipient), a local cultivar of superior quality, gave rise to regeneration of several lines of shoots, which failed to root despite enormous endeavors. Ploidy analysis via flow cytometry and chromosome counting showed that four selected shoots were either diploid, triploid or tetraploid. Random amplified polymorphism DNA (RAPD) and amplified fragment length polymorphism (AFLP) confirmed the shoots, irrespective of their ploidy level, as putative somatic hybrids. Cleaved amplified polymorphism sequences (CAPS) demonstrated that the shoots predominantly got their cytoplasmic components, in terms of chloroplast (cp) and mitochondrion DNA, from Jincheng, along with possible recombination of cpDNA in some shoot lines. The current data indicated that UV-based asymmetric fusion could also be employed in citrus somatic hybridization with the intention of creating novel germplasms, which may provide an alternative approach for cultivar improvement.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Xu XY,Hu ZY,Li JF,Liu JH,Deng XXdoi
10.1007/s00299-007-0350-7subject
Has Abstractpub_date
2007-08-01 00:00:00pages
1263-73issue
8eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0756-5
更新日期：2009-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232932
更新日期：1994-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-014-1678-4
更新日期：2014-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0873-1
更新日期：2010-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-010-0922-9
更新日期：2010-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231961
更新日期：1994-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193145
更新日期：1991-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232816
更新日期：1993-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2068-x
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-004-0911-y
更新日期：2005-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0276-5
更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00232106
更新日期：1991-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-005-0936-x
更新日期：2005-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1667-7
更新日期：2014-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269739
更新日期：1987-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232404
更新日期：1990-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0770-7
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journal_title：Plant cell reports
pub_type： 杂志文章
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193724
更新日期：1995-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269538
更新日期：1988-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234146
更新日期：1994-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1670-z
更新日期：2014-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0952-x
更新日期：2005-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2004-0
更新日期：2016-09-01 00:00:00
abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00241946
更新日期：1993-01-01 00:00:00
