Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons.

Abstract:

:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chimeric gene was delivered to the cotyledons via particle bombardment 900 PSI. Zygotic cotyledons from six soybean varieties were tested for transient expression of the β-glucuronidase gene. The level of reporter gene expression differed between genotypes. The genotypes could be classified as high, fair or poor expressors. Cotyledons from different genotypes were then bombarded at 650, 900 or 1100 PSI. GUS expression varied among genotypes independently of the pressure of bombardment. Finally, the ability of cotyledons to express the reporter gene depended on the developmental stage of the seed from which it was excised with the younger stage being the least responsive. However, genotypic specific expression remained after controlling for developmental stage of the cotyledons.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Moore PJ,Moore AJ,Collins GB

doi

10.1007/BF00234510

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

556-60

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

13

pub_type

杂志文章
  • Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.

    abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0276-5

    authors: Wang DJ,Guo AG,Li DR,Tian JH,Huang F,Sun GL

    更新日期:2007-05-01 00:00:00

  • DNA methylation changes in clonally propagated oil palm.

    abstract:KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02561-9

    authors: Sarpan N,Taranenko E,Ooi SE,Low EL,Espinoza A,Tatarinova TV,Ong-Abdullah M

    更新日期:2020-09-01 00:00:00

  • Plant regeneration from callus and protoplasts of Brassica nigra (IC 257) through somatic embryogenesis.

    abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232265

    authors: Gupta V,Agnihotri A,Jagannathan V

    更新日期:1990-12-01 00:00:00

  • Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones.

    abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0836-5

    authors: Kim ST,Kim SG,Hwang DH,Kang SY,Koo SC,Cho MJ,Kang KY

    更新日期:2004-10-01 00:00:00

  • Abscisic acid-regulated Glb1 transient expression in cultured maize P3377 cells.

    abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050459

    authors: Liu S,Kriz A,Duncan D,Widholm J

    更新日期:1998-05-01 00:00:00

  • Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L.

    abstract::Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232160

    authors: Hippolyte I,Marin B,Baccou JC,Jonard R

    更新日期:1992-04-01 00:00:00

  • Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice.

    abstract:KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1758-0

    authors: Nahampun HN,Bosworth B,Cunnick J,Mogler M,Wang K

    更新日期:2015-06-01 00:00:00

  • Embryogenesis in suspension cultures of Datura innoxia Mill.

    abstract::In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233065

    authors: Sharma VK,Jethwani V,Kothari SL

    更新日期:1993-08-01 00:00:00

  • Effect of 1-Aminocyclopropane-1-carboxylic acid and aminoethoxyvinylglycine on ethylene emanation and somatic embryogenesis from orchardgrass leaf cultures.

    abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272059

    authors: Songstad DD,Petracek PD,Sams CE,Conger BV

    更新日期:1989-03-01 00:00:00

  • Chloroplast DNA synthesis in light and dark grown cultured Nicotiana tabacum cells as determined by molecular hybridization.

    abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269202

    authors: Cannon G,Heinhorst S,Siedlecki J,Weissbach A

    更新日期:1985-04-01 00:00:00

  • Fruit quality and DNA methylation are affected by parental order in reciprocal crosses of tomato.

    abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02624-x

    authors: Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

    更新日期:2021-01-01 00:00:00

  • Callus production from leaf protoplasts of various cultivars of bean (Phaseolus vulgaris L.).

    abstract::High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269250

    authors: Crepy L,Barros LM,Valente VR

    更新日期:1986-04-01 00:00:00

  • A promoter derived from taro bacilliform badnavirus drives strong expression in transgenic banana and tobacco plants.

    abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0621-x

    authors: Yang IC,Iommarini JP,Becker DK,Hafner GJ,Dale JL,Harding RM

    更新日期:2003-08-01 00:00:00

  • Introduction of a chimeric gene encoding an oryzacystatin-β-glucuronidase fusion protein into rice protoplasts and regeneration of transformed plants.

    abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193714

    authors: Hosoyama H,Irie K,Abe K,Arai S

    更新日期:1995-12-01 00:00:00

  • Propagation of fern (Nephrolepis) through tissue culture.

    abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272634

    authors: Padhya MA,Mehta AR

    更新日期:1982-12-01 00:00:00

  • Somatic embryogenesis and in vitro flowering of 3 species of bamboo.

    abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231624

    authors: Rout GR,Das P

    更新日期:1994-09-01 00:00:00

  • AtHSP17.8 overexpression in transgenic lettuce gives rise to dehydration and salt stress resistance phenotypes through modulation of ABA-mediated signaling.

    abstract:KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-013-1506-2

    authors: Kim DH,Xu ZY,Hwang I

    更新日期:2013-12-01 00:00:00

  • Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid.

    abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0614-9

    authors: Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

    更新日期:2003-06-01 00:00:00

  • Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

    abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1112-0

    authors: Gao ZM,Li CL,Peng ZH

    更新日期:2011-11-01 00:00:00

  • Screening of diploid Medicago sativa germplasm for somatic embryogenesis.

    abstract::Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a hi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269379

    authors: Meijer EG,Brown DC

    更新日期:1985-10-01 00:00:00

  • Low night temperature acclimation of Phalaenopsis.

    abstract::The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1021-2

    authors: Pollet B,Vanhaecke L,Dambre P,Lootens P,Steppe K

    更新日期:2011-06-01 00:00:00

  • Extracellular accumulation of high specific-activity peroxidase by cell suspension cultures of cowpea.

    abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232092

    authors: Moreno OA,Vazquez-Duhalt R,Nolasco H

    更新日期:1990-07-01 00:00:00

  • Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato.

    abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1073-3

    authors: Sorkina A,Bardosh G,Liu YZ,Fridman I,Schlizerman L,Zur N,Or E,Goldschmidt EE,Blumwald E,Sadka A

    更新日期:2011-09-01 00:00:00

  • Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis).

    abstract:KEY MESSAGE:A homologous gene of MPEC from Phyllostachys edulis was isolated and characterized. Its overexpression in Arabidopsis thaliana increased chlorophyll concentration and photosynthesis efficiency, indicating it is involved in chlorophyll biosynthesis. Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC)...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1846-1

    authors: Yang L,Lou Y,Peng Z,Zhao H,Sun H,Gao Z

    更新日期:2015-11-01 00:00:00

  • Production of gynogenic haploids of Hordeum vulgare L.

    abstract::Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00239094

    authors: Castillo AM,Cistué L

    更新日期:1993-01-01 00:00:00

  • Increased formation of cinnamoyl putrescines by fedbatch fermentation of cell suspension cultures of Nicotiana tabacum.

    abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270221

    authors: Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

    更新日期:1984-02-01 00:00:00

  • Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures.

    abstract::A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232422

    authors: Su RC,Rudert ML,Hodges TK

    更新日期:1992-12-01 00:00:00

  • NADPH-protochlorophyllide oxidoreductase: Reciprocal regulation in mono- and dicotyledonean plants.

    abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269229

    authors: Meyer G,Bliedung H,Kloppstech K

    更新日期:1983-02-01 00:00:00

  • Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture.

    abstract::The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050520

    authors: Redha A,Attia T,Büter B,Saisingtong S,Stamp P,Schmid JE

    更新日期:1998-09-01 00:00:00

  • A method for inflorescence proliferation.

    abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0571-3

    authors: Lin CS,Chen CT,Lin CC,Chang WC

    更新日期:2003-06-01 00:00:00