Abstract:
:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chimeric gene was delivered to the cotyledons via particle bombardment 900 PSI. Zygotic cotyledons from six soybean varieties were tested for transient expression of the β-glucuronidase gene. The level of reporter gene expression differed between genotypes. The genotypes could be classified as high, fair or poor expressors. Cotyledons from different genotypes were then bombarded at 650, 900 or 1100 PSI. GUS expression varied among genotypes independently of the pressure of bombardment. Finally, the ability of cotyledons to express the reporter gene depended on the developmental stage of the seed from which it was excised with the younger stage being the least responsive. However, genotypic specific expression remained after controlling for developmental stage of the cotyledons.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Moore PJ,Moore AJ,Collins GBdoi
10.1007/BF00234510subject
Has Abstractpub_date
1994-07-01 00:00:00pages
556-60issue
10eissn
0721-7714issn
1432-203Xjournal_volume
13pub_type
杂志文章abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0276-5
更新日期:2007-05-01 00:00:00
abstract:KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02561-9
更新日期:2020-09-01 00:00:00
abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232265
更新日期:1990-12-01 00:00:00
abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0836-5
更新日期:2004-10-01 00:00:00
abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050459
更新日期:1998-05-01 00:00:00
abstract::Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232160
更新日期:1992-04-01 00:00:00
abstract:KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1758-0
更新日期:2015-06-01 00:00:00
abstract::In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233065
更新日期:1993-08-01 00:00:00
abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272059
更新日期:1989-03-01 00:00:00
abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269202
更新日期:1985-04-01 00:00:00
abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02624-x
更新日期:2021-01-01 00:00:00
abstract::High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269250
更新日期:1986-04-01 00:00:00
abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0621-x
更新日期:2003-08-01 00:00:00
abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193714
更新日期:1995-12-01 00:00:00
abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272634
更新日期:1982-12-01 00:00:00
abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
abstract:KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1506-2
更新日期:2013-12-01 00:00:00
abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1112-0
更新日期:2011-11-01 00:00:00
abstract::Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a hi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269379
更新日期:1985-10-01 00:00:00
abstract::The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1021-2
更新日期:2011-06-01 00:00:00
abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232092
更新日期:1990-07-01 00:00:00
abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1073-3
更新日期:2011-09-01 00:00:00
abstract:KEY MESSAGE:A homologous gene of MPEC from Phyllostachys edulis was isolated and characterized. Its overexpression in Arabidopsis thaliana increased chlorophyll concentration and photosynthesis efficiency, indicating it is involved in chlorophyll biosynthesis. Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC)...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1846-1
更新日期:2015-11-01 00:00:00
abstract::Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239094
更新日期:1993-01-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract::A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232422
更新日期:1992-12-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050520
更新日期:1998-09-01 00:00:00
abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0571-3
更新日期:2003-06-01 00:00:00