Plant regeneration from callus and protoplasts of Brassica nigra (IC 257) through somatic embryogenesis.

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：:Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...

journal_title：Plant cell reports

authors： Jain AK,Dandin SB,Sengupta K

更新日期：1990-05-01 00:00:00

abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...

journal_title：Plant cell reports

authors： Phan TT,Sun B,Niu JQ,Tan QL,Li J,Yang LT,Li YR

更新日期：2016-09-01 00:00:00

abstract：:Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...

journal_title：Plant cell reports

authors： Ford KG

更新日期：1990-01-01 00:00:00

abstract：:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...

journal_title：Plant cell reports

authors： Guri A,Zelcer A,Izhar S

更新日期：1984-12-01 00:00:00

abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...

journal_title：Plant cell reports

authors： Mishra MK,Tiwari S,Misra P

更新日期：2021-02-01 00:00:00

abstract：:Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

journal_title：Plant cell reports

authors： Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

更新日期：2010-08-01 00:00:00

abstract：:We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...

journal_title：Plant cell reports

authors： Qiao J,Kuroda H,Hayashi T,Sakai F

更新日期：1998-01-01 00:00:00

abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...

journal_title：Plant cell reports

authors： Dmitriev A,Djatsok J,Grodzinsky D

更新日期：1996-09-01 00:00:00

abstract：: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...

journal_title：Plant cell reports

authors： Becker DK,Dugdale B,Smith MK,Harding RM,Dale JL

更新日期：2000-01-01 00:00:00

abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...

journal_title：Plant cell reports

authors： Baek KH,Skinner DZ

更新日期：2006-03-01 00:00:00

abstract：KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...

journal_title：Plant cell reports

authors： Xu C,Xia C,Xia Z,Zhou X,Huang J,Huang Z,Liu Y,Jiang Y,Casteel S,Zhang C

更新日期：2018-12-01 00:00:00

abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...

journal_title：Plant cell reports

authors： Utomo HS,Croughan SS,Croughan TP

更新日期：1995-01-01 00:00:00

abstract：KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...

journal_title：Plant cell reports

authors： Kim DH,Xu ZY,Hwang I

更新日期：2013-12-01 00:00:00

abstract：:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

journal_title：Plant cell reports

authors： Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

更新日期：1990-08-01 00:00:00

abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

journal_title：Plant cell reports

authors： Pan G,Zhou Y,Fowke LC,Wang H

更新日期：2004-10-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) activity, contents of phenolic acids and ethylene production during the lag-phase, and contents of phenolic acids at the late exponential phase, showed significant differences in embryogenic (EC) and non-embryogenic (NEC) suspension cultures of Medicago sativa L. Maximum PAL activity ...

journal_title：Plant cell reports

authors： Cvikrová M,Meravý L,Macháčková I,Eder J

更新日期：1991-08-01 00:00:00

abstract：:We report here the successful establishment of callus, cell and root cultures from explants of in-vitro-grown plantlets of the soapwort Saponaria officinalis L. The production of saporin in the different tissue systems was evaluated by determining the capability of crude extracts to inactivate protein synthesis and by...

journal_title：Plant cell reports

authors： Di Cola A,Di Domenico C,Poma A,Spanò L

更新日期：1997-11-01 00:00:00

abstract：:Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...

journal_title：Plant cell reports

authors： Vergara R,Verde F,Pitto L,Lo Schiavo F,Terzi M

更新日期：1990-05-01 00:00:00

abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...

journal_title：Plant cell reports

authors： Gan D,Zhang J,Jiang H,Jiang T,Zhu S,Cheng B

更新日期：2010-11-01 00:00:00

abstract：:KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...

journal_title：Plant cell reports

authors： Nguyen KT,Towler MJ,Weathers PJ

更新日期：2013-02-01 00:00:00

abstract：KEY MESSAGE:SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieve...

journal_title：Plant cell reports

authors： Guo JE,Hu Z,Yu X,Li A,Li F,Wang Y,Tian S,Chen G

更新日期：2018-01-01 00:00:00

abstract：:Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...

journal_title：Plant cell reports

authors： Yun LJ,Chen WL

更新日期：2011-07-01 00:00:00

abstract：:We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...

journal_title：Plant cell reports

authors： Li L,Hou X,Tsuge T,Ding M,Aoyama T,Oka A,Gu H,Zhao Y,Qu LJ

更新日期：2008-03-01 00:00:00

abstract：:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...

journal_title：Plant cell reports

authors： Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

更新日期：2006-10-01 00:00:00

abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...

journal_title：Plant cell reports

authors： Tabaeizadeh Z,Campeau N

更新日期：1992-03-01 00:00:00

abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...

journal_title：Plant cell reports

authors： Godwin I,Todd G,Ford-Lloyd B,Newbury HJ

更新日期：1991-04-01 00:00:00

abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

journal_title：Plant cell reports

authors： Schmidt M,Parrott W

更新日期：2001-07-01 00:00:00

abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...

journal_title：Plant cell reports

authors： Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

更新日期：2000-11-01 00:00:00

abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...

journal_title：Plant cell reports

authors： Soares EL,Lima MLB,Nascimento JRS,Soares AA,Coutinho ÍAC,Campos FAP

更新日期：2017-11-01 00:00:00