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Stable transformation of papaya via microprojectile bombardment.

Abstract：

:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10 mg 1(-1) 2,4-D, 400 mg 1(-1) glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150 mg 1(-1) kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots. Leafy shoots were produced six to nine months following bombardment. Tissues from 13 of these isolates were assayed for NPTII activity, and 10 were positive. Six out of 15 isolates assayed for GUS expression were positive. Three isolates were positive for both NPTII and GUS.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

doi

10.1007/BF00232177

subject

Has Abstract

pub_date

1990-08-01 00:00:00

pages

189-94

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

9

pub_type

杂志文章

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