Abstract:
:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric NPTII and GUS genes. All tissue types were cultured prior to and following bombardment on half-strength MS medium supplemented with 10 mg 1(-1) 2,4-D, 400 mg 1(-1) glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150 mg 1(-1) kanamycin sulfate, ten putative transgenic isolates produced somatic embryos and five regenerated leafy shoots. Leafy shoots were produced six to nine months following bombardment. Tissues from 13 of these isolates were assayed for NPTII activity, and 10 were positive. Six out of 15 isolates assayed for GUS expression were positive. Three isolates were positive for both NPTII and GUS.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JCdoi
10.1007/BF00232177subject
Has Abstractpub_date
1990-08-01 00:00:00pages
189-94issue
4eissn
0721-7714issn
1432-203Xjournal_volume
9pub_type
杂志文章abstract:KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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journal_title:Plant cell reports
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doi:10.1007/s00299-004-0910-z
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journal_title:Plant cell reports
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
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abstract::In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269152
更新日期:1983-10-01 00:00:00
abstract::Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...
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pub_type: 杂志文章
doi:10.1007/s00299-005-0937-9
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1006-1
更新日期:2011-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0224-4
更新日期:2007-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239094
更新日期:1993-01-01 00:00:00
abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050753
更新日期:2000-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1846-1
更新日期:2015-11-01 00:00:00
abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0809-8
更新日期:2004-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232737
更新日期:1995-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1725-1
更新日期:2015-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0922-9
更新日期:2010-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0579-9
更新日期:2008-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1990-07-01 00:00:00
abstract::The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0917-0
更新日期:2005-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237128
更新日期:1993-03-01 00:00:00
abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233813
更新日期:1991-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0712-4
更新日期:2009-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272536
更新日期:1988-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1422-5
更新日期:2013-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234710
更新日期:1993-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233638
更新日期:1995-01-01 00:00:00