Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses.

abstract：:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...

journal_title：Plant cell reports

authors： Nakagawa K,Konagai A,Fukui H,Tabata M

更新日期：1984-12-01 00:00:00

abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

journal_title：Plant cell reports

authors： Gu XF,Zhang JR

更新日期：2005-03-01 00:00:00

abstract：:Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium and by temperature. The highest concentration of essential oils was obtained when regenerated flowers were...

journal_title：Plant cell reports

authors： Hosokawa K,Fukunaga Y

更新日期：1995-06-01 00:00:00

abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...

journal_title：Plant cell reports

authors： Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

更新日期：2015-10-01 00:00:00

abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

journal_title：Plant cell reports

authors： Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

更新日期：1989-02-01 00:00:00

abstract：UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...

journal_title：Plant cell reports

authors： Padmesh P,Mukunthakumar S,Vineesh PS,Skaria R,Hari Kumar K,Krishnan PN

更新日期：2012-09-01 00:00:00

abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...

journal_title：Plant cell reports

authors： Gao J,Lee JM,An G

更新日期：1991-12-01 00:00:00

abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...

journal_title：Plant cell reports

authors： Samaj J,Salaj T,Matúsová R,Salaj J,Takác T,Samajová O,Volkmann D

更新日期：2008-02-01 00:00:00

abstract：:The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...

journal_title：Plant cell reports

authors： Teixeira da Silva JA,Tsavkelova EA,Ng TB,Parthibhan S,Dobránszki J,Cardoso JC,Rao MV,Zeng S

更新日期：2015-10-01 00:00:00

abstract：:Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...

journal_title：Plant cell reports

authors： Havel L,Novák FJ

更新日期：1981-08-01 00:00:00

abstract：:We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that A...

journal_title：Plant cell reports

authors： van Roekel JS,Damm B,Melchers LS,Hoekema A

更新日期：1993-09-01 00:00:00

abstract：: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...

journal_title：Plant cell reports

authors： Nobre J,Davey MR,Lazzeri PA,Cannell ME

更新日期：2000-10-01 00:00:00

abstract：:Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...

journal_title：Plant cell reports

authors： Buenrostro-Nava MT,Ling PP,Finer JJ

更新日期：2006-09-01 00:00:00

abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

journal_title：Plant cell reports

authors： Caillot S,Rosiau E,Laplace C,Thomasset B

更新日期：2009-03-01 00:00:00

abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...

journal_title：Plant cell reports

authors： Wakasa Y,Kasai A,Yamazaki M,Tabei Y,Tsuyama M,Igarashi T,Okazaki T,Yamamoto K,Fujihara H,Kanno A,Noro O,Harada T,Akada S

更新日期：2020-11-01 00:00:00

abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...

journal_title：Plant cell reports

authors： Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ES

更新日期：1997-09-01 00:00:00

abstract：:Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeri...

journal_title：Plant cell reports

authors： Vallés MP,Lasa JM

更新日期：1994-01-01 00:00:00

abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...

journal_title：Plant cell reports

authors： Lee S,Hong JC,Jeon WB,Chung YS,Sung S,Choi D,Joung YH,Oh BJ

更新日期：2009-10-01 00:00:00

abstract：:Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...

journal_title：Plant cell reports

authors： Yang IC,Iommarini JP,Becker DK,Hafner GJ,Dale JL,Harding RM

更新日期：2003-08-01 00:00:00

abstract：:A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted cop...

journal_title：Plant cell reports

authors： Melander M,Kamnert I,Happstadius I,Liljeroth E,Bryngelsson T

更新日期：2006-09-01 00:00:00

abstract：:The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...

journal_title：Plant cell reports

authors： Okamoto A,Kishine S,Hirosawa T,Nakazono A

更新日期：1996-06-01 00:00:00

abstract：:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...

journal_title：Plant cell reports

authors： Ueno K,Fukunaga Y,Arisumi K

更新日期：1996-11-01 00:00:00

abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

journal_title：Plant cell reports

authors： Schumacher HM,Gundlach H,Fiedler F,Zenk MH

更新日期：1987-12-01 00:00:00

abstract：:The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glu...

journal_title：Plant cell reports

authors： Chibbar RN,Kartha KK,Datla RS,Leung N,Caswell K,Mallard CS,Steinhauer L

更新日期：1993-07-01 00:00:00

abstract：:Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

journal_title：Plant cell reports

authors： Szabados L,Gaggero C

更新日期：1985-08-01 00:00:00

abstract：:Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...

journal_title：Plant cell reports

authors： Toivonen L,Laakso S,Rosenqvist H

更新日期：1992-07-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

journal_title：Plant cell reports

authors： Singh R,Jwa NS

更新日期：2013-06-01 00:00:00

abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...

journal_title：Plant cell reports

authors： Hasan MM,Kim HS,Jeon JH,Kim SH,Moon B,Song JY,Shim SH,Baek KH

更新日期：2014-06-01 00:00:00

abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

journal_title：Plant cell reports

authors： Nussbaumer P,Kapétanidis I,Christen P

更新日期：1998-03-01 00:00:00

abstract：:The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...

journal_title：Plant cell reports

authors： Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

更新日期：1995-10-01 00:00:00