The stability of foreign protein production in genetically modified plant cells.

abstract：:Eukaryotic gene expression is regulated at least by two processes, RNA interference at the post-transcriptional level and chromatin modification at the transcriptional level. Distinct small RNAs (approximately 21-24 nucleotides; sRNAs) were demonstrated to play vital roles in facilitating gene silencing. In plants, th...

journal_title：Plant cell reports

authors： Qian Y,Cheng Y,Cheng X,Jiang H,Zhu S,Cheng B

更新日期：2011-07-01 00:00:00

abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

journal_title：Plant cell reports

authors： Murphy JF,Kyle MM

更新日期：1994-04-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...

journal_title：Plant cell reports

authors： Mino M,Oka M,Tasaka Y,Iwabuchi M

更新日期：2003-09-01 00:00:00

abstract：:Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus t...

journal_title：Plant cell reports

authors： Heyenga AG,Lucas JA,Dewick PM

更新日期：1990-11-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...

journal_title：Plant cell reports

authors： Peng R,Bian Z,Zhou L,Cheng W,Hai N,Yang C,Yang T,Wang X,Wang C

更新日期：2016-11-01 00:00:00

abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...

journal_title：Plant cell reports

authors： Sonia,Saini R,Singh RP,Jaiwal PK

更新日期：2007-02-01 00:00:00

abstract：:Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

journal_title：Plant cell reports

authors： Gao ZM,Li CL,Peng ZH

更新日期：2011-11-01 00:00:00

abstract：:Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...

journal_title：Plant cell reports

authors： Schwechheimer C,Schwager K

更新日期：2004-11-01 00:00:00

abstract：: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...

journal_title：Plant cell reports

authors： Gürel F,Gözükirmizi N

更新日期：2000-07-01 00:00:00

abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

journal_title：Plant cell reports

authors： Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

更新日期：2012-06-01 00:00:00

abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

journal_title：Plant cell reports

authors： Nussbaumer P,Kapétanidis I,Christen P

更新日期：1998-03-01 00:00:00

abstract：:Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...

journal_title：Plant cell reports

authors： Ford KG

更新日期：1990-01-01 00:00:00

abstract：KEY MESSAGE:In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic...

journal_title：Plant cell reports

authors： Winnicki K,Żabka A,Bernasińska J,Matczak K,Maszewski J

更新日期：2015-06-01 00:00:00

abstract：:The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anti-clinal cell divisions. Differentiation of the protoderm cell layer by periclin...

journal_title：Plant cell reports

authors： Ramakrishnan K,Gnanam R,Sivakumar P,Manickam A

更新日期：2005-11-01 00:00:00

abstract：: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...

journal_title：Plant cell reports

authors： Hirai D,Sakai A

更新日期：1999-12-01 00:00:00

abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

journal_title：Plant cell reports

authors： Evans DA,Gamborg OL

更新日期：1982-04-01 00:00:00

abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...

journal_title：Plant cell reports

authors： Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

更新日期：1988-12-01 00:00:00

abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...

journal_title：Plant cell reports

authors： Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers L

更新日期：2003-11-01 00:00:00

abstract：KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...

journal_title：Plant cell reports

authors： Lv F,Li P,Zhang R,Li N,Guo W

更新日期：2016-04-01 00:00:00

abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...

journal_title：Plant cell reports

authors： Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ES

更新日期：1997-09-01 00:00:00

abstract：:The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

journal_title：Plant cell reports

authors： Hernández I,Celestino C,Alegre J,Toribio M

更新日期：2003-04-01 00:00:00

abstract：:We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...

journal_title：Plant cell reports

authors： Qiao J,Kuroda H,Hayashi T,Sakai F

更新日期：1998-01-01 00:00:00

abstract：:Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...

journal_title：Plant cell reports

authors： Bhat SR,Ford-Lloyd BV,Callow JA

更新日期：1985-12-01 00:00:00

abstract：:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...

journal_title：Plant cell reports

authors： Nishimaki T,Nozue M

更新日期：1985-10-01 00:00:00

abstract：:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...

journal_title：Plant cell reports

authors： Davis DG,Olson PA,Stolzenberg RL

更新日期：1988-06-01 00:00:00

abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

journal_title：Plant cell reports

authors： Ozias-Akins P

更新日期：1989-04-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：KEY MESSAGE:When fused to " Pr AlSAP " promoter, transcripts of gusA exhibited similar accumulation patterns in transgenic rice as AlSAP transcripts in A. littoralis. Pr AlSAP can be used for engineering abiotic stress tolerance. We previously showed that ectopic expression of a stress-associated protein gene from Aelu...

journal_title：Plant cell reports

authors： Ben-Saad R,Meynard D,Ben-Romdhane W,Mieulet D,Verdeil JL,Al-Doss A,Guiderdoni E,Hassairi A

更新日期：2015-10-01 00:00:00