Abstract：
: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4 °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were dehydrated with a highly concentrated vitrification solution (PVS2 solution) for 3 h at 0 °C prior to a plunge into liquid nitrogen. Successfully encapsulated vitrified meristems developed shoots within a week after plating without intermediary callus formation. The average rate of shoot formation amounted to nearly 90%. This procedure was successfully applied to other Mentha species. It was also confirmed that encapsulated vitrified meristems produced a much higher rate of shoot formation than the encapsulated dried meristems. Thus, this revised encapsulation vitrification method appears promising for the cryopreservation of mint and other germplasm.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Hirai D,Sakai Adoi
10.1007/s002990050725subject
Has Abstractpub_date
1999-12-01 00:00:00pages
150-155issue
2eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050725journal_volume
19pub_type
杂志文章abstract：:Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272634
更新日期：1982-12-01 00:00:00
abstract：:The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anti-clinal cell divisions. Differentiation of the protoderm cell layer by periclin...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0966-4
更新日期：2005-11-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
abstract：:KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1355-4
更新日期：2013-02-01 00:00:00
abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0911-z
更新日期：2010-11-01 00:00:00
abstract：:A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193734
更新日期：1995-12-01 00:00:00
abstract：:Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00267652
更新日期：1981-08-01 00:00:00
abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050491
更新日期：1998-07-01 00:00:00
abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1591-x
更新日期：2014-05-01 00:00:00
abstract：UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1247-7
更新日期：2012-07-01 00:00:00
abstract：:Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233291
更新日期：1994-11-01 00:00:00
abstract：:A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050359
更新日期：1997-12-01 00:00:00
abstract：:In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 year...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0413-9
更新日期：2007-11-01 00:00:00
abstract：:Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193724
更新日期：1995-12-01 00:00:00
abstract：:Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1735-z
更新日期：2015-04-01 00:00:00
abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2217-x
更新日期：2018-01-01 00:00:00
abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2184-2
更新日期：2017-11-01 00:00:00
abstract：KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02638-5
更新日期：2021-02-01 00:00:00
abstract：:Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235303
更新日期：1993-12-01 00:00:00
abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193714
更新日期：1995-12-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1725-1
更新日期：2015-03-01 00:00:00
abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269987
更新日期：1990-04-01 00:00:00
abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2079-7
更新日期：2017-02-01 00:00:00
abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270062
更新日期：1990-03-01 00:00:00
abstract：:A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0321-z
更新日期：2007-07-01 00:00:00
abstract：:A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0736-0
更新日期：2004-03-01 00:00:00
abstract：:Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0845-5
更新日期：2010-06-01 00:00:00
abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02571-7
更新日期：2020-11-01 00:00:00
abstract：:Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233325
更新日期：1994-02-01 00:00:00
abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1773-1
更新日期：2015-07-01 00:00:00
