Transformation of floral organs with GFP in Medicago truncatula.

Abstract:

: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

doi

10.1007/s002999900168

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

647-653

issue

7

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002999900168

journal_volume

19

pub_type

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