Abstract:
: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh THdoi
10.1007/s002999900168subject
Has Abstractpub_date
2000-06-01 00:00:00pages
647-653issue
7eissn
0721-7714issn
1432-203Xpii
10.1007/s002999900168journal_volume
19pub_type
杂志文章abstract::The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0917-0
更新日期:2005-06-01 00:00:00
abstract::Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193155
更新日期:1991-09-01 00:00:00
abstract:KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1667-7
更新日期:2014-11-01 00:00:00
abstract:KEY MESSAGE:Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02634-9
更新日期:2021-02-01 00:00:00
abstract::Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231961
更新日期:1994-05-01 00:00:00
abstract::A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050359
更新日期:1997-12-01 00:00:00
abstract:KEY MESSAGE:Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis. ABSTRACT:Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1480-8
更新日期:2013-11-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract::The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233425
更新日期:1993-10-01 00:00:00
abstract::From the in vitro hairy root cultures of a Datura candida hybrid, 19 tropane alkaloids have been identified using capillary gas-liquid chromatography and mass spectrometry. As in the parent plants, scopolamine is the major alkaloid. Two hitherto undescribed alkaloids have been detected and their structure tentatively ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231559
更新日期:1990-07-01 00:00:00
abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2176-2
更新日期:2017-10-01 00:00:00
abstract:KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2039-2
更新日期:2016-11-01 00:00:00
abstract::We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050378
更新日期:1998-01-01 00:00:00
abstract::Cell suspension cultures of chickpea (Cicer arietinum L.) were established from cultivars ILC 3279 and ILC 1929, resistant and susceptible towards the chickpea pathogenic fungus Ascochyta rabiei. The two cell culture lines possess identical growth properties and show high accumulation of the isoflavones biochanin A an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269739
更新日期:1987-02-01 00:00:00
abstract::Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0550-0
更新日期:2003-02-01 00:00:00
abstract::Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-004-0858-z
更新日期:2004-11-01 00:00:00
abstract::Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0437-1
更新日期:2008-01-01 00:00:00
abstract::Chloroplast-nucleus communication takes place via processes called anterograde and retrograde signaling pathways. Discovery of the retrograde signaling pathways from the chloroplasts to the nucleus also raised an intriguing proposition that chloroplasts may serve as environmental sensors since multitudes of environmen...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02383-4
更新日期:2019-07-01 00:00:00
abstract:KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2184-2
更新日期:2017-11-01 00:00:00
abstract::A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050426
更新日期:1998-04-01 00:00:00
abstract::We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide ge...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269646
更新日期:1986-12-01 00:00:00
abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0200-z
更新日期:2006-12-01 00:00:00
abstract::Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0250-2
更新日期:2007-04-01 00:00:00
abstract::In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Muta...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0922-9
更新日期:2010-12-01 00:00:00
abstract:: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000219
更新日期:2000-10-01 00:00:00
abstract::Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated u...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02482-2
更新日期:2020-01-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0254-y
更新日期:2007-03-01 00:00:00
abstract::Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1139-2
更新日期:2012-01-01 00:00:00
abstract::An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234700
更新日期:1993-05-01 00:00:00