Abstract：
:Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their fast polar tip growth. Plasma membrane labelling of pollen tubes, grown in suspension, was achieved by the incorporation of a fluorescently modified phospholipid, 1,2-bis-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoyl)-sn-glycero-3-phosphocholine (20 microM) and measured with a confocal laser-scanning microscope. Time course experiments revealed a highly localised and relatively fast plasma membrane retrieval below the tip within the first 5 min after phospholipid application. The retrieved fluorescent plasma membrane was quickly re-integrated into parts of the endomembrane pool and then redistributed to the pollen tube base and very tip of the apex, with the exception of the cortical endoplasmic reticulum (ER) and the mitochondria even after 1-h incubation period. Low temperature (10 degrees C) and the actin filament depolymerizing cytochalasin D (2 microM) completely abolished plasma membrane retrieval, whereas the microtubule destabilizing herbicide oryzalin (1 microM) had no effect. Our results provide strong support for a highly localised endocytotic pathway in tobacco pollen tubes. Passive uptake of bis-Bodipy FL C(11)-phosphocholine by mere penetration can be excluded. It is a valuable alternative to the styryl dyes often used in endocytotic studies, and may also be used to follow lipid turnover because membrane flow of labelled membranes occurs apparently not in a default manner as ascertained by its fast distribution.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Lisboa S,Scherer GE,Quader Hdoi
10.1007/s00299-007-0437-1subject
Has Abstractpub_date
2008-01-01 00:00:00pages
21-8issue
1eissn
0721-7714issn
1432-203Xjournal_volume
27pub_type
杂志文章abstract：:Since plant root systems capture both water and nutrients essential for the formation of crop yield, there has been renewed biotechnological focus on root system improvement. Although water and nutrient uptake can be facilitated by membrane proteins known as aquaporins and nutrient transporters, respectively, there is...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-011-1005-2
更新日期：2011-05-01 00:00:00
abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050753
更新日期：2000-04-01 00:00:00
abstract：:Intertribal somatic hybrids between Brassica napus (2n = 38, AACC) and a dye and medicinal plant Isatis indigotica (2n = 14, II) were obtained by fusions of mesophyll protoplasts. From a total of 237 calli, only one symmetric hybrid (S2) and five asymmetric hybrids (As1, As4, As6, As7 and As12) were established in the...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0712-4
更新日期：2009-07-01 00:00:00
abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1768-y
更新日期：2015-05-01 00:00:00
abstract：:Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
abstract：:Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716854
更新日期：1989-02-01 00:00:00
abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270062
更新日期：1990-03-01 00:00:00
abstract：:The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0604-y
更新日期：2003-04-01 00:00:00
abstract：KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2190-4
更新日期：2017-11-01 00:00:00
abstract：KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2088-6
更新日期：2017-02-01 00:00:00
abstract：:Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272107
更新日期：1990-05-01 00:00:00
abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1819-4
更新日期：2015-10-01 00:00:00
abstract：:High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232691
更新日期：1992-08-01 00:00:00
abstract：:Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1052-8
更新日期：2011-08-01 00:00:00
abstract：:A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269538
更新日期：1988-10-01 00:00:00
abstract：KEY MESSAGE:In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1752-6
更新日期：2015-06-01 00:00:00
abstract：: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. milti...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002999900166
更新日期：2000-06-01 00:00:00
abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000237
更新日期：2000-11-01 00:00:00
abstract：:The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236096
更新日期：1993-07-01 00:00:00
abstract：:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233028
更新日期：1991-05-01 00:00:00
abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272770
更新日期：1987-12-01 00:00:00
abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1832-7
更新日期：2015-10-01 00:00:00
abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1230-3
更新日期：2012-06-01 00:00:00
abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00778536
更新日期：1989-04-01 00:00:00
abstract：:We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050378
更新日期：1998-01-01 00:00:00
abstract：:A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0321-z
更新日期：2007-07-01 00:00:00
abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050321
更新日期：1997-09-01 00:00:00
abstract：:Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269116
更新日期：1986-06-01 00:00:00
abstract：:Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234710
更新日期：1993-05-01 00:00:00
