Dissected effect of a transit peptide of the ADP-glucose pyrophosphorylase gene from sweetpotato (ibAGP2) in increasing foreign protein accumulation.

Abstract:

:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the transit peptide in elevating foreign protein accumulation in the transgenic plant. beta-glucuronidase protein levels were determined at three different developmental stages and in assorted tissues. TP2 dramatically elevated GUS protein accumulation regardless of developmental stage, but the level of the enhancing effect was developmental stage-dependent. This enhancing effect was strongest at the seedling stage (16-fold) and relatively moderate at the vegetative (tenfold) and reproductive (11-fold) stages. TP2 also elevated GUS protein accumulation to varying degrees (4 to 19-fold) in assorted tissues, with the effect being highest in the primary inflorescence stem and petiole (19-fold) and weakest in the root (fourfold). Although TP2 also increased GUS mRNA levels, the increased levels were not large enough to account for the elevated GUS protein levels, suggesting that the enhancing effect of TP2 does not solely result from increased levels of transcripts. Taken together, our results reveal that the TP2 significantly increased the levels of protein accumulation and that its effectiveness was developmental stage- and tissue-type-dependent in transgenic Arabidopsis. Possible differential targeting efficiencies of different transit peptides are discussed.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Kwak MS,Oh MJ,Paek KH,Shin JS,Bae JM

doi

10.1007/s00299-008-0563-4

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

1359-67

issue

8

eissn

0721-7714

issn

1432-203X

journal_volume

27

pub_type

杂志文章
  • Thidiazuron-induced plant regeneration from hypocotyl cultures of St. John's wort (Hypericum perforatum. cv 'Anthos').

    abstract:: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050776

    authors: Murch SJ,Choffe KL,Victor JMR,Slimmon TY,KrishnaRaj S,Saxena PK

    更新日期:2000-05-01 00:00:00

  • Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles.

    abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0254-y

    authors: Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

    更新日期:2007-03-01 00:00:00

  • Quantitative trait loci from identification to exploitation for crop improvement.

    abstract::Advancement in the field of genetics and genomics after the discovery of Mendel's laws of inheritance has led to map the genes controlling qualitative and quantitative traits in crop plant species. Mapping of genomic regions controlling the variation of quantitatively inherited traits has become routine after the adve...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-017-2127-y

    authors: Kumar J,Gupta DS,Gupta S,Dubey S,Gupta P,Kumar S

    更新日期:2017-08-01 00:00:00

  • Asymbiotic in vitro seed propagation of Dendrobium.

    abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1829-2

    authors: Teixeira da Silva JA,Tsavkelova EA,Ng TB,Parthibhan S,Dobránszki J,Cardoso JC,Rao MV,Zeng S

    更新日期:2015-10-01 00:00:00

  • Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures.

    abstract::A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232422

    authors: Su RC,Rudert ML,Hodges TK

    更新日期:1992-12-01 00:00:00

  • Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones.

    abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1183-y

    authors: Prabu G,Prasad DT

    更新日期:2012-04-01 00:00:00

  • Fruit quality and DNA methylation are affected by parental order in reciprocal crosses of tomato.

    abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02624-x

    authors: Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

    更新日期:2021-01-01 00:00:00

  • Selection for NaCl tolerance in cell cultures of Medicago sativa and recovery of plants from a NaCl-tolerant cell line.

    abstract::Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269335

    authors: Smith MK,McComb JA

    更新日期:1983-06-01 00:00:00

  • An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants.

    abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0920-5

    authors: Gu XF,Zhang JR

    更新日期:2005-03-01 00:00:00

  • Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration.

    abstract::The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation-vitrification (EnVi) and encapsulation-dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, r...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0936-x

    authors: Wang Q,Laamanen J,Uosukainen M,Valkonen JP

    更新日期:2005-07-01 00:00:00

  • A Streptomyces chitosanase is active in transgenic tobacco.

    abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193724

    authors: El Quakfaoui S,Potvin C,Brzezinski R,Asselin A

    更新日期:1995-12-01 00:00:00

  • Protoplast-derived streptomycin resistant plants of the forage legume Onobrychis viciifolia Scop. (sainfoin).

    abstract::Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269636

    authors: Hamill JD,Ahuja PS,Davey MR,Cocking EC

    更新日期:1986-12-01 00:00:00

  • Hydrogen sulfide enhances nitric oxide-induced tolerance of hypoxia in maize (Zea mays L.).

    abstract:KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2037-4

    authors: Peng R,Bian Z,Zhou L,Cheng W,Hai N,Yang C,Yang T,Wang X,Wang C

    更新日期:2016-11-01 00:00:00

  • Regulated proteolysis and plant development.

    abstract::Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-004-0858-z

    authors: Schwechheimer C,Schwager K

    更新日期:2004-11-01 00:00:00

  • Ingestion of transgenic carrots expressing the Escherichia coli heat-labile enterotoxin B subunit protects mice against cholera toxin challenge.

    abstract::Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0439-z

    authors: Rosales-Mendoza S,Soria-Guerra RE,López-Revilla R,Moreno-Fierros L,Alpuche-Solís AG

    更新日期:2008-01-01 00:00:00

  • Abscisic acid-regulated Glb1 transient expression in cultured maize P3377 cells.

    abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050459

    authors: Liu S,Kriz A,Duncan D,Widholm J

    更新日期:1998-05-01 00:00:00

  • Expression of human growth hormone in transgenic rice cell suspension culture.

    abstract::Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0514-0

    authors: Kim TG,Baek MY,Lee EK,Kwon TH,Yang MS

    更新日期:2008-05-01 00:00:00

  • Calmodulin-binding protein CBP60g is a positive regulator of both disease resistance and drought tolerance in Arabidopsis.

    abstract:UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-012-1247-7

    authors: Wan D,Li R,Zou B,Zhang X,Cong J,Wang R,Xia Y,Li G

    更新日期:2012-07-01 00:00:00

  • The Arabidopsis thaliana hypocotyl, a model to identify and study control mechanisms of cellular expansion.

    abstract::Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-014-1591-x

    authors: Boron AK,Vissenberg K

    更新日期:2014-05-01 00:00:00

  • Isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato.

    abstract::Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269369

    authors: Nishimaki T,Nozue M

    更新日期:1985-10-01 00:00:00

  • Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus.

    abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269721

    authors: Yamamoto H,Nakagawa K,Fukui H,Tabata M

    更新日期:1986-02-01 00:00:00

  • Variability in tissue cultures of Choisya ternata. Alkaloid accumulation in protoclones and aggregate clones obtained from established strains.

    abstract::Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269565

    authors: Tremouillaux-Guiller J,Andreu F,Creche J,Chenieux JC,Rideau M

    更新日期:1987-10-01 00:00:00

  • Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.

    abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0276-5

    authors: Wang DJ,Guo AG,Li DR,Tian JH,Huang F,Sun GL

    更新日期:2007-05-01 00:00:00

  • Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses.

    abstract::Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0937-9

    authors: Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

    更新日期:2005-08-01 00:00:00

  • Cell reabsorption in the Euphorbia dulcis endosperm.

    abstract::Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231924

    authors: Muccifora S,Gori P

    更新日期:1996-05-01 00:00:00

  • Programmed-cell-death hallmarks in incompatible pollen and papillar stigma cells of Olea europaea L. under free pollination.

    abstract::Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0845-5

    authors: Serrano I,Pelliccione S,Olmedilla A

    更新日期:2010-06-01 00:00:00

  • A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight.

    abstract::Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0059-4

    authors: Mackintosh CA,Garvin DF,Radmer LE,Heinen SJ,Muehlbauer GJ

    更新日期:2006-04-01 00:00:00

  • In vitro propagation ofAtractylodes lancea.

    abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF02441005

    authors: Hiraoka N,Yamada N,Kodama T,Tomita Y

    更新日期:1984-06-01 00:00:00

  • Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification.

    abstract:: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050725

    authors: Hirai D,Sakai A

    更新日期:1999-12-01 00:00:00

  • Genetic transformation of Populus nigra by Agrobacterium tumefaciens.

    abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233315

    authors: Confalonieri M,Balestrazzi A,Bisoffi S

    更新日期:1994-02-01 00:00:00