Programmed-cell-death hallmarks in incompatible pollen and papillar stigma cells of Olea europaea L. under free pollination.

Abstract:

:Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause of pollen-growth arrest. As far as the olive is concerned, no data have been published concerning the mechanisms involved in hindering the growth of pollen tubes in incompatible pollen. Thus, we have studied olive pistils excised from freely pollinated flowers at different stages before and during the progamic phase using different cytochemical techniques, including trypan blue staining. To discover whether the elimination of incompatible pollen might be associated to PCD, we applied different tests to the excised pistils: (1) TUNEL assay; (2) DNA degradation analysis; (3) detection of caspase-3-like activity. Once we had determined that PCD was involved in pollen selection after free pollination, we conducted experiments after controlled pollination in pistils excised from flowers: (a) developing in the absence of pollen; (b) pollinated with sterile pollen that does not germinate; (c) self-pollinated; (d) pollinated with compatible pollen. Our results demonstrate that the growth of tubes in incompatible pollen is halted in the stylar area in a way that suggests the intervention of PCD. Furthermore, any pollen, even if sterile, seemed to accelerate PCD in papillar cells in the olive.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Serrano I,Pelliccione S,Olmedilla A

doi

10.1007/s00299-010-0845-5

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

561-72

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

29

pub_type

杂志文章
  • Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons.

    abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234510

    authors: Moore PJ,Moore AJ,Collins GB

    更新日期:1994-07-01 00:00:00

  • The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis.

    abstract::We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0458-9

    authors: Li L,Hou X,Tsuge T,Ding M,Aoyama T,Oka A,Gu H,Zhao Y,Qu LJ

    更新日期:2008-03-01 00:00:00

  • Hairy roots of Datura candida×D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis.

    abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050415

    authors: Nussbaumer P,Kapétanidis I,Christen P

    更新日期:1998-03-01 00:00:00

  • The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

    abstract::Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00235354

    authors: Godwin I,Todd G,Ford-Lloyd B,Newbury HJ

    更新日期:1991-04-01 00:00:00

  • The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition.

    abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234368

    authors: Toivonen L,Laakso S,Rosenqvist H

    更新日期:1992-07-01 00:00:00

  • Influence of benzyladenine on condensed tannin formation and callus growth in cultures from sainfoin (Onobrychis viccifolia Scop.) cotyledons.

    abstract::Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269314

    authors: Lees GL

    更新日期:1988-05-01 00:00:00

  • Correction to: Improved insect resistance against Spodoptera litura in transgenic sweetpotato by overexpressing Cry1Aa toxin.

    abstract::In Materials and method section, a sweetpotato variety "Taizhong-6" (China national number 2013003) should be renamed as Ayamurasaki". ...

    journal_title:Plant cell reports

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s00299-019-02487-x

    authors: Zhong Y,Ahmed S,Deng G,Fan W,Zhang P,Wang H

    更新日期:2020-01-01 00:00:00

  • Identification and characterization of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families in maize.

    abstract::Eukaryotic gene expression is regulated at least by two processes, RNA interference at the post-transcriptional level and chromatin modification at the transcriptional level. Distinct small RNAs (approximately 21-24 nucleotides; sRNAs) were demonstrated to play vital roles in facilitating gene silencing. In plants, th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1046-6

    authors: Qian Y,Cheng Y,Cheng X,Jiang H,Zhu S,Cheng B

    更新日期:2011-07-01 00:00:00

  • In vitro propagation ofMimosa tenuiflora (Willd.) Poiret, a Mexican medicinal tree.

    abstract::Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01275455

    authors: Villarreal ML,Rojas G

    更新日期:1996-11-01 00:00:00

  • Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material.

    abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269825

    authors: Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

    更新日期:1986-08-01 00:00:00

  • Effects of cadmium stress on the antioxidant system and chlorophyll fluorescence characteristics of two Taxodium clones.

    abstract:KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2327-0

    authors: Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

    更新日期:2018-11-01 00:00:00

  • Small heat shock protein LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily.

    abstract::Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1106-y

    authors: Mu C,Wang S,Zhang S,Pan J,Chen N,Li X,Wang Z,Liu H

    更新日期:2011-10-01 00:00:00

  • Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.).

    abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050481

    authors: Ayabe M,Sumi S

    更新日期:1998-07-01 00:00:00

  • Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.).

    abstract:KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1670-z

    authors: Wan F,Pan Y,Li J,Chen X,Pan Y,Wang Y,Tian S,Zhang X

    更新日期:2014-12-01 00:00:00

  • Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera.

    abstract::Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232537

    authors: Burnett L,Yarrow S,Huang B

    更新日期:1992-05-01 00:00:00

  • Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.).

    abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233055

    authors: Nielsen KA,Larsen E,Knudsen E

    更新日期:1993-08-01 00:00:00

  • High-efficiency biolistic co-transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) containing npt-II and antimicrobial peptide genes.

    abstract::A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0682-x

    authors: Vidal JR,Kikkert JR,Wallace PG,Reisch BI

    更新日期:2003-11-01 00:00:00

  • In vivo nitrate reductase activity in dark- and light-grown sugarcane callus.

    abstract::The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270207

    authors: Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

    更新日期:1984-08-01 00:00:00

  • Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon : II. A new Medium for the production of shikonin derivatives.

    abstract::An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effect...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269273

    authors: Fujita Y,Hara Y,Suga C,Morimoto T

    更新日期:1981-12-01 00:00:00

  • Chloroplast DNA synthesis in light and dark grown cultured Nicotiana tabacum cells as determined by molecular hybridization.

    abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269202

    authors: Cannon G,Heinhorst S,Siedlecki J,Weissbach A

    更新日期:1985-04-01 00:00:00

  • Production of disease-free encapsulated buds of Zingiber officinale Rosc.

    abstract::Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233325

    authors: Sharma TR,Singh BM,Chauhan RS

    更新日期:1994-02-01 00:00:00

  • In planta 2,3,5 truodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants.

    abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01890855

    authors: Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

    更新日期:1996-12-01 00:00:00

  • A Streptomyces chitosanase is active in transgenic tobacco.

    abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193724

    authors: El Quakfaoui S,Potvin C,Brzezinski R,Asselin A

    更新日期:1995-12-01 00:00:00

  • A simple system for pea transformation.

    abstract::The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01142315

    authors: Bean SJ,Gooding PS,Mullincaux PM,Davies DR

    更新日期:1997-05-01 00:00:00

  • Introduction of foreign genes into Pharbitis nil calli using a vector derived from Agrobacterium pTi.

    abstract::Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00274124

    authors: Araki T,Hirano H,Naito S,Komeda Y

    更新日期:1989-05-01 00:00:00

  • Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

    abstract::Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234579

    authors: Warkentin TD,McHughen A

    更新日期:1991-12-01 00:00:00

  • Rapid micropropagation for Mucuna pruriens f. pruriens L.

    abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193734

    authors: Chattopadhyay S,Datta SK,Mahato SB

    更新日期:1995-12-01 00:00:00

  • Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids?

    abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269126

    authors: Aviv D,Chen R,Galun E

    更新日期:1986-06-01 00:00:00

  • Polyphenoloxidase-activity and -activation in embryogenic and non-embryogenic suspension cultures of Euphorbia pulcherrima.

    abstract::The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234048

    authors: Grotkass C,Lieberei R,Preil W

    更新日期:1995-04-01 00:00:00

  • Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.).

    abstract::A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231799

    authors: Chang YF,Wang WC,Warfield CY,Nguyen HT,Wong JR

    更新日期:1991-03-01 00:00:00