Abstract：
:Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. After 21 days the fresh weight of calli treated with BAP was much greater than the control and appeared highest at the 0.6 mg/L level. Fresh weight was reduced at high BAP levels (2 to 8 mg/L) but still remained well above the control. The formation of tannin-filled cells was genotype-specific but occurred in all treatments with BAP. After 21 days in culture, fresh weight and tannin formation increased with the BAP level at the lower BAP concentrations. After 31 days, the growth rate slowed in the control and the lower BAP concentration, but continued at a high rate in the remaining treatments; the number of tannin-filled cells appeared to decline. The results show that in sainfoin callus culture, BAP induces the formation of condensed tannin, a secondary metabolite, and concomitantly produces a high growth rate.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Lees GLdoi
10.1007/BF00269314subject
Has Abstractpub_date
1988-05-01 00:00:00pages
166-9issue
3eissn
0721-7714issn
1432-203Xjournal_volume
7pub_type
杂志文章abstract：:A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0697-z
更新日期：2009-06-01 00:00:00
abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0756-5
更新日期：2009-10-01 00:00:00
abstract：: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050702
更新日期：1999-11-01 00:00:00
abstract：:The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1028-8
更新日期：2011-07-01 00:00:00
abstract：:Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232412
更新日期：1992-12-01 00:00:00
abstract：:Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270076
更新日期：1989-10-01 00:00:00
abstract：:A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232727
更新日期：1995-07-01 00:00:00
abstract：KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1667-7
更新日期：2014-11-01 00:00:00
abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050426
更新日期：1998-04-01 00:00:00
abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050491
更新日期：1998-07-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1725-1
更新日期：2015-03-01 00:00:00
abstract：:In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0091-4
更新日期：2006-06-01 00:00:00
abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0614-9
更新日期：2003-06-01 00:00:00
abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231832
更新日期：1992-02-01 00:00:00
abstract：:Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0672-z
更新日期：2003-09-01 00:00:00
abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0836-5
更新日期：2004-10-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：:Shoot regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.) was tested on MS basal medium supplemented with four different growth regulators. Regeneration frequencies for medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 60 μM 4amino-3, 5,6-picolinic acid (piclora...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232655
更新日期：1995-08-01 00:00:00
abstract：:Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0514-0
更新日期：2008-05-01 00:00:00
abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269555
更新日期：1987-10-01 00:00:00
abstract：:An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233776
更新日期：1994-12-01 00:00:00
abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1164-1
更新日期：2012-02-01 00:00:00
abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0659-9
更新日期：2003-08-01 00:00:00
abstract：:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0657-z
更新日期：2009-03-01 00:00:00
abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0534-9
更新日期：2008-07-01 00:00:00
abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0092-3
更新日期：2006-04-01 00:00:00
abstract：KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1928-0
更新日期：2016-04-01 00:00:00
abstract：:Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233325
更新日期：1994-02-01 00:00:00
abstract：:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231809
更新日期：1991-03-01 00:00:00
abstract：:A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050446
更新日期：1998-04-01 00:00:00
