Abstract:
:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of nitrate reductase activity followed Michaelian kinetics. The apparent Km values for NADH were 0.083 mM and 0.20 mM, respectively, for the dark- and the light-grown callus. In vivo nitrate reductase activity in green sugarcane leaves (field grown) was unaffected by NADH in the reaction system. Under the standard conditions of assay up to 60% of the NADH penetrated into the sugarcane callus within 2 min. No penetration of NADH into the sugarcane leaf discs was, however, recorded under identical conditions.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AFdoi
10.1007/BF00270207subject
Has Abstractpub_date
1984-08-01 00:00:00pages
138-41issue
4eissn
0721-7714issn
1432-203Xjournal_volume
3pub_type
杂志文章abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
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journal_title:Plant cell reports
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