In vivo nitrate reductase activity in dark- and light-grown sugarcane callus.

Abstract:

:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of nitrate reductase activity followed Michaelian kinetics. The apparent Km values for NADH were 0.083 mM and 0.20 mM, respectively, for the dark- and the light-grown callus. In vivo nitrate reductase activity in green sugarcane leaves (field grown) was unaffected by NADH in the reaction system. Under the standard conditions of assay up to 60% of the NADH penetrated into the sugarcane callus within 2 min. No penetration of NADH into the sugarcane leaf discs was, however, recorded under identical conditions.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

doi

10.1007/BF00270207

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

138-41

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

3

pub_type

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