Efficient callus formation and plant regeneration from leaves of oats (Avena sativa L.).

abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

journal_title：Plant cell reports

authors： Castillo AM,Cistué L

更新日期：1993-01-01 00:00:00

abstract：:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...

journal_title：Plant cell reports

authors： Passey AJ,Barrett KJ,James DJ

更新日期：2003-01-01 00:00:00

abstract：:The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...

journal_title：Plant cell reports

authors： Redha A,Attia T,Büter B,Saisingtong S,Stamp P,Schmid JE

更新日期：1998-09-01 00:00:00

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：:The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anti-clinal cell divisions. Differentiation of the protoderm cell layer by periclin...

journal_title：Plant cell reports

authors： Ramakrishnan K,Gnanam R,Sivakumar P,Manickam A

更新日期：2005-11-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....

journal_title：Plant cell reports

authors： Kim SW,Ban SH,Chung HJ,Choi DW,Choi PS,Yoo OJ,Liu JR

更新日期：2004-02-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

journal_title：Plant cell reports

authors： Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

更新日期：2007-04-01 00:00:00

abstract：:The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...

journal_title：Plant cell reports

authors： Decendit A,Petit G,Andreu F,Doireau P,Mérillon JM,Rideau M

更新日期：1993-10-01 00:00:00

abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...

journal_title：Plant cell reports

authors： Crepy L,Barros LM,Valente VR

更新日期：1986-04-01 00:00:00

abstract：:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

journal_title：Plant cell reports

authors： Lyznik LA,Gordon-Kamm WJ,Tao Y

更新日期：2003-06-01 00:00:00

abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

journal_title：Plant cell reports

authors： Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

更新日期：1997-09-01 00:00:00

abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...

journal_title：Plant cell reports

authors： Grotkass C,Lieberei R,Preil W

更新日期：1995-04-01 00:00:00

abstract：:Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...

journal_title：Plant cell reports

authors： Yooyongwech S,Sugaya S,Sekozawa Y,Gemma H

更新日期：2009-11-01 00:00:00

abstract：:Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...

journal_title：Plant cell reports

authors： Jain AK,Dandin SB,Sengupta K

更新日期：1990-05-01 00:00:00

abstract：:A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...

journal_title：Plant cell reports

authors： Mohan ML,Krishnamurthy KV

更新日期：1998-06-01 00:00:00

abstract：:KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...

journal_title：Plant cell reports

authors： Terashima M,Fukukita A,Kodama R,Miki H,Suzuki M,Ikegami M,Tamura N,Yasuda A,Morikawa M,Matsumura S

更新日期：2013-03-01 00:00:00

abstract：:In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...

journal_title：Plant cell reports

authors： Ssymank V

更新日期：1983-10-01 00:00:00

abstract：:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1982-06-01 00:00:00

abstract：KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

journal_title：Plant cell reports

authors： An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

更新日期：2020-08-01 00:00:00

abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...

journal_title：Plant cell reports

authors： Snyman SJ,Hajari E,Watt MP,Lu Y,Kridl JC

更新日期：2015-05-01 00:00:00

abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

journal_title：Plant cell reports

authors： Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

更新日期：1998-04-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

journal_title：Plant cell reports

authors： Guo B,Gao M,Liu CZ

更新日期：2007-03-01 00:00:00

abstract：:The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...

journal_title：Plant cell reports

authors： Belivanis T,Doré C

更新日期：1986-10-01 00:00:00

abstract：:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...

journal_title：Plant cell reports

authors： Choi YE,Yang DC,Kim HS,Choi KT

更新日期：1997-10-01 00:00:00

abstract：:Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...

journal_title：Plant cell reports

authors： Lee-Parsons CW,Royce AJ

更新日期：2006-06-01 00:00:00

abstract：:Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...

journal_title：Plant cell reports

authors： Winicov I

更新日期：1991-12-01 00:00:00

abstract：:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...

journal_title：Plant cell reports

authors： Araki T,Hirano H,Naito S,Komeda Y

更新日期：1989-05-01 00:00:00

abstract：KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...

journal_title：Plant cell reports

authors： Muñoz-Bodnar A,Perez-Quintero AL,Gomez-Cano F,Gil J,Michelmore R,Bernal A,Szurek B,Lopez C

更新日期：2014-11-01 00:00:00