Characterization of salt tolerant alfalfa (Medicago sativa L.) plants regenerated from salt tolerant cell lines.

Abstract:

:Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cellular salt tolerance characteristic can be passaged through the regenerated plants, since callus cultures initiated from immature ovaries of the salt tolerant regenerated plants are salt tolerant without additional selection on 1% NaCl. Several of these "second generation" callus cultures have been regenerated to produce vigorous plants which maintain the salt tolerance characteristic. The tolerance phenotype appears dominant in seeds obtained from self fertilization of the tolerant plants. The regenerated salt tolerant plants are therefore a valuable source as genotypes in plant breeding for salt tolerance and isolation, identification and manipulation of genes which confer salt tolerance in alfalfa.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Winicov I

doi

10.1007/BF00232511

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

561-4

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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