Abstract:
:Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzyme can be glycosylated in the seeds of transgenic plants and retain a high stability during storage. These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Peng RH,Yao QH,Xiong AS,Cheng ZM,Li Ydoi
10.1007/s00299-005-0036-ykeywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
124-32issue
2eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
杂志文章abstract::Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231583
更新日期:1996-09-01 00:00:00
abstract:KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1644-1
更新日期:2014-10-01 00:00:00
abstract::Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-016-2017-8
更新日期:2016-09-01 00:00:00
abstract:KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2190-4
更新日期:2017-11-01 00:00:00
abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
更新日期:2005-03-01 00:00:00
abstract::In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0942-5
更新日期:2011-03-01 00:00:00
abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193734
更新日期:1995-12-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272634
更新日期:1982-12-01 00:00:00
abstract::Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0770-7
更新日期:2009-11-01 00:00:00
abstract::Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716668
更新日期:1989-06-01 00:00:00
abstract:KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1892-8
更新日期:2016-02-01 00:00:00
abstract:: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050014
更新日期:2000-01-01 00:00:00
abstract::Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232412
更新日期:1992-12-01 00:00:00
abstract::This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0687-5
更新日期:2003-11-01 00:00:00
abstract::In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269152
更新日期:1983-10-01 00:00:00
abstract::Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1118-7
更新日期:2011-11-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract::The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0530-4
更新日期:2003-01-01 00:00:00
abstract::KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1368-z
更新日期:2013-03-01 00:00:00
abstract::A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050576
更新日期:1998-12-01 00:00:00
abstract::Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276666
更新日期:1987-04-01 00:00:00
abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269116
更新日期:1986-06-01 00:00:00
abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
abstract::An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238594
更新日期:1995-03-01 00:00:00
abstract::Suspension cultures derived from Lycium chinense Miller seedlings produced significant amounts of a hepatoprotective cerebroside. Callus was induced from the stem of aseptic seedlings of L. chinense and maintained on MS solid media supplemented with 1.0 ppm 2,4-D and 0.1 ppm kinetin. Suspension cultures were establish...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050566
更新日期:1998-12-01 00:00:00
abstract::A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050469
更新日期:1998-06-01 00:00:00
abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0571-3
更新日期:2003-06-01 00:00:00
abstract::A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0909-6
更新日期:2010-11-01 00:00:00
abstract:KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1895-5
更新日期:2016-02-01 00:00:00