Abstract:
:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that were collected at the end of June yielded embryogenic tissue at the highest frequency. Embryogenic tissues that had proliferated in liquid medium included small and loosely packed cells and elongating or elongated cells. We used ten cell lines to determine the optimal medium for the development of somatic embryos. Induced somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots. Gibberellin A3 in the germination medium had a positive effect on both the elongation of hypocotyls and the survival of seedlings. The frequencies of induction and germination of somatic embryos differed among the cell lines examined. Most of the seedlings grew normally. This system of somatic embryogenesis required 4-5 months for the regeneration of C. japonica plantlets from immature zygotic embryos.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Igasaki T,Sato T,Akashi N,Mohri T,Maruyama E,Kinoshita I,Walter C,Shinohara Kdoi
10.1007/s00299-003-0687-5keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
239-43issue
4eissn
0721-7714issn
1432-203Xjournal_volume
22pub_type
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