Abstract:
:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indicated that Mol7 possesses greater potential than B73 to acquire thermal tolerance. Poly(A)(+) RNA, extracted from leaf blades, was translated in vitro in the presence of (35)S-methionine and the translation products separated by twodimensional gel electrophoresis. Major genotypic differences were observed in the translation products. Mo 17 synthesized twelve unique heat shock proteins in the 15-18 kD range, but B73 synthesized only three unique heat shock proteins in the same range. DNA polymorphisms were observed between the maize lines using (32)P labeled heat shock protein gene probes.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Jorgensen JA,Weng J,Ho TH,Nguyen HTdoi
10.1007/BF00233096subject
Has Abstractpub_date
1992-10-01 00:00:00pages
576-80issue
11eissn
0721-7714issn
1432-203Xjournal_volume
11pub_type
杂志文章abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232608
更新日期:1991-10-01 00:00:00
abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269212
更新日期:1985-04-01 00:00:00
abstract::The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270194
更新日期:1984-10-01 00:00:00
abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1768-y
更新日期:2015-05-01 00:00:00
abstract::To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236381
更新日期:1992-11-01 00:00:00
abstract::The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0444-2
更新日期:2008-01-01 00:00:00
abstract:: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000219
更新日期:2000-10-01 00:00:00
abstract::Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268613
更新日期:1986-10-01 00:00:00
abstract::We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232858
更新日期:1990-10-01 00:00:00
abstract::Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1118-7
更新日期:2011-11-01 00:00:00
abstract::The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0539-4
更新日期:2008-07-01 00:00:00
abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2068-x
更新日期:2017-01-01 00:00:00
abstract::Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269636
更新日期:1986-12-01 00:00:00
abstract::Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0657-z
更新日期:2009-03-01 00:00:00
abstract::Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0109-y
更新日期:2006-06-01 00:00:00
abstract::Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232127
更新日期:1990-06-01 00:00:00
abstract:KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02572-6
更新日期:2020-11-01 00:00:00
abstract::Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00820203
更新日期:1990-01-01 00:00:00
abstract::Reduced glutathione (GSH) was applied to prevent browning in shoot tip explants of apple (Malus pumila Mill.). Development was compared between shoot tips treated either by (a) dipping into 0.1 mM GSH solution prior to culture (dip treatment), (b) dipping into the GSH solution and transferring to a medium containing 0...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050449
更新日期:1998-05-01 00:00:00
abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571295
更新日期:1985-05-01 00:00:00
abstract::Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0733-z
更新日期:2009-09-01 00:00:00
abstract::Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233415
更新日期:1993-10-01 00:00:00
abstract::The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0974-x
更新日期:2011-04-01 00:00:00
abstract::Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233291
更新日期:1994-11-01 00:00:00
abstract::Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233028
更新日期:1991-05-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1725-1
更新日期:2015-03-01 00:00:00
abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0230-6
更新日期:2007-03-01 00:00:00
abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0621-x
更新日期:2003-08-01 00:00:00
abstract::It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272049
更新日期:1989-03-01 00:00:00
abstract:: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. milti...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900166
更新日期:2000-06-01 00:00:00