Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.


KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different signaling pathways involved in hormone signaling, plant growth, development, and both abiotic and biotic stress responses. In this study, we describe the potato CDPK/CRK multigene family; bioinformatic analysis allowed us to identify 20 new CDPK isoforms, three CDPK-related kinases (CRKs), and a CDPK-like kinase. Phylogenetic analysis indicated that 26 StCDPKs can be classified into four groups, whose members are predicted to undergo different acylation patterns and exhibited diverse expression levels in different tissues and in response to various stimuli. With the aim of characterizing those members that are particularly involved in plant-pathogen interaction, we focused on StCDPK7. Tissue expression profile revealed that StCDPK7 transcript levels are high in swollen stolons, roots, and mini tubers. Moreover, its expression is induced upon Phytophthora infestans infection in systemic leaves. Transient expression assays showed that StCDPK7 displays a cytosolic/nuclear localization in spite of having a predicted chloroplast transit peptide. The recombinant protein, StCDPK7:6xHis, is an active Ca2+-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase, an enzyme involved in plant defense response. The analysis of the potato CDPK family provides the first step towards the identification of CDPK isoforms involved in biotic stress. StCDPK7 emerges as a relevant player that could be manipulated to deploy disease resistance in potato crops.


Plant Cell Rep


Plant cell reports


Fantino E,Segretin ME,Santin F,Mirkin FG,Ulloa RM




Has Abstract


2017-07-01 00:00:00














  • Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

    abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gao ZM,Li CL,Peng ZH

    更新日期:2011-11-01 00:00:00

  • Regeneration of horseradish hairy roots incited by Agrobacterium rhizogenes infection.

    abstract::Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Noda T,Tanaka N,Mano Y,Nabeshima S,Ohkawa H,Matsui C

    更新日期:1987-07-01 00:00:00

  • Fruit quality and DNA methylation are affected by parental order in reciprocal crosses of tomato.

    abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

    更新日期:2021-01-01 00:00:00

  • Agrobacterium tumefaciens mediated transfer of Phaseolus vulgaris alpha-amylase inhibitor-1 gene into mungbean Vigna radiata (L.) Wilczek using bar as selectable marker.

    abstract::Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sonia,Saini R,Singh RP,Jaiwal PK

    更新日期:2007-02-01 00:00:00

  • Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum.

    abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Mino M,Oka M,Tasaka Y,Iwabuchi M

    更新日期:2003-09-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Virus-induced gene silencing (VIGS)-mediated functional characterization of two genes involved in lignocellulosic secondary cell wall formation.

    abstract:KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Pandey SK,Nookaraju A,Fujino T,Pattathil S,Joshi CP

    更新日期:2016-11-01 00:00:00

  • Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification.

    abstract:: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hirai D,Sakai A

    更新日期:1999-12-01 00:00:00

  • A method for inflorescence proliferation.

    abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Lin CS,Chen CT,Lin CC,Chang WC

    更新日期:2003-06-01 00:00:00

  • Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.).

    abstract:KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wan F,Pan Y,Li J,Chen X,Pan Y,Wang Y,Tian S,Zhang X

    更新日期:2014-12-01 00:00:00

  • Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana.

    abstract::Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

    更新日期:2007-11-01 00:00:00

  • Will the EU stay out of step with science and the rest of the world on plant breeding innovation?

    abstract::Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated u...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Jorasch P

    更新日期:2020-01-01 00:00:00

  • Localized endocytosis in tobacco pollen tubes: visualisation and dynamics of membrane retrieval by a fluorescent phospholipid.

    abstract::Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Lisboa S,Scherer GE,Quader H

    更新日期:2008-01-01 00:00:00

  • Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells.

    abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ingersoll JC,Heutte TM,Owens LD

    更新日期:1996-08-01 00:00:00

  • Propagation of fern (Nephrolepis) through tissue culture.

    abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Padhya MA,Mehta AR

    更新日期:1982-12-01 00:00:00

  • Gene silencing studies in the gymnosperm species Pinus radiata.

    abstract::A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

    更新日期:2005-05-01 00:00:00

  • Reverse transcriptase domain sequences from Mungbean (Vigna radiata) LTR retrotransposons: sequence characterization and phylogenetic analysis.

    abstract::The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Dixit A,Ma KH,Yu JW,Cho EG,Park YJ

    更新日期:2006-03-01 00:00:00

  • Voltage-dependent anion channels: their roles in plant defense and cell death.

    abstract::The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Kusano T,Tateda C,Berberich T,Takahashi Y

    更新日期:2009-09-01 00:00:00

  • Differences in pectic polysaccharides between carrot embryogenic and non-embryogenic calli.

    abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kikuchi A,Satoh S,Nakamura N,Fujii T

    更新日期:1995-02-01 00:00:00

  • High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter.

    abstract:KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Honma Y,Yamakawa T

    更新日期:2019-11-01 00:00:00

  • Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

    abstract::Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sikdar SR,Chatterjee G,Das S,Sen SK

    更新日期:1987-12-01 00:00:00

  • Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco.

    abstract:KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Phan TT,Sun B,Niu JQ,Tan QL,Li J,Yang LT,Li YR

    更新日期:2016-09-01 00:00:00

  • Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf.

    abstract::The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kim MK,Lee BS,In JG,Sun H,Yoon JH,Yang DC

    更新日期:2006-06-01 00:00:00

  • Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

    abstract::An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Watad AA,Kochba M,Nissim A,Gaba V

    更新日期:1995-03-01 00:00:00

  • Organic nitrogen stimulates caulogenesis from hypocotyl callus of Phyllanthus fraternus.

    abstract::Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rajasubramaniam S,Pardha Saradhi P

    更新日期:1994-08-01 00:00:00

  • Transformation of Actinidia eriantha: a potential species for functional genomics studies in Actinidia.

    abstract::Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen D

    更新日期:2006-05-01 00:00:00

  • Additional virulence genes and sonication enhance Agrobacterium tumefaciens-mediated loblolly pine transformation.

    abstract::Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Tang W

    更新日期:2003-02-01 00:00:00

  • Influence of benzyladenine on condensed tannin formation and callus growth in cultures from sainfoin (Onobrychis viccifolia Scop.) cotyledons.

    abstract::Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Lees GL

    更新日期:1988-05-01 00:00:00

  • Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis.

    abstract::Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gilmour DM,Davey MR,Cocking EC

    更新日期:1989-05-01 00:00:00

  • Metabolic engineering of isoflavone genistein in Brassica napus with soybean isoflavone synthase.

    abstract::Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Li X,Qin JC,Wang QY,Wu X,Lang CY,Pan HY,Gruber MY,Gao MJ

    更新日期:2011-08-01 00:00:00