Abstract：
:Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a high 2,4-D concentration and a two-step sequence without exposure to a high 2,4-D concentration. Considerable variation for callus proliferation was observed. In general, the diploid M. sativa accessions showed poor regenerability and it was not possible to correlate high regeneration frequencies with a particular germplasm source. It was, however, possible to identify regenerable genotypes in all four subspecies. One falcata accession produced somatic embryos on the callus induction media at high frequencies. This response was also obtained with a few genotypes from one xvaria accession. All regenerable plants were maintained as shoot cultures and were able to form somatic embryos on petiole-derived calli.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Meijer EG,Brown DCdoi
10.1007/BF00269379subject
Has Abstractpub_date
1985-10-01 00:00:00pages
285-8issue
5eissn
0721-7714issn
1432-203Xjournal_volume
4pub_type
杂志文章abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272760
更新日期：1988-12-01 00:00:00
abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232312
更新日期：1993-11-01 00:00:00
abstract：:Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0571-3
更新日期：2003-06-01 00:00:00
abstract：:To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1067-1
更新日期：2011-08-01 00:00:00
abstract：KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2356-8
更新日期：2019-02-01 00:00:00
abstract：:Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to dir...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232170
更新日期：1992-04-01 00:00:00
abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232946
更新日期：1994-06-01 00:00:00
abstract：:Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272972
更新日期：1988-01-01 00:00:00
abstract：:Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270076
更新日期：1989-10-01 00:00:00
abstract：:Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a sear...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0910-z
更新日期：2005-06-01 00:00:00
abstract：KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultiva...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1699-z
更新日期：2015-02-01 00:00:00
abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233813
更新日期：1991-11-01 00:00:00
abstract：:Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0439-z
更新日期：2008-01-01 00:00:00
abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270062
更新日期：1990-03-01 00:00:00
abstract：:Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268613
更新日期：1986-10-01 00:00:00
abstract：: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000219
更新日期：2000-10-01 00:00:00
abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0638-2
更新日期：2009-03-01 00:00:00
abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2184-2
更新日期：2017-11-01 00:00:00
abstract：KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02535-x
更新日期：2020-07-01 00:00:00
abstract：: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000295
更新日期：2001-02-01 00:00:00
abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050763
更新日期：2000-04-01 00:00:00
abstract：:The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0664-0
更新日期：2009-04-01 00:00:00
abstract：:Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269240
更新日期：1986-04-01 00:00:00
abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00778536
更新日期：1989-04-01 00:00:00
abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1832-7
更新日期：2015-10-01 00:00:00
abstract：:KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1374-1
更新日期：2013-03-01 00:00:00
abstract：:Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0594-x
更新日期：2008-11-01 00:00:00
abstract：:An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effect...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269273
更新日期：1981-12-01 00:00:00
abstract：:The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890855
更新日期：1996-12-01 00:00:00
abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272049
更新日期：1989-03-01 00:00:00
