Abstract:
: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used for histochemical localization of β-glucuronidase (GUS) activity in transgenic plants (Petunia hybrida, Arabidopsis thaliana, and Nicotiana tabacum) that contained the GUS reporter system. Because the cleavage of the β-glucuronide results in the liberation of an insoluble Sudan dye, Sudan substrates gave no diffusion artifacts as described for the commonly used 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-gluc). A comparison of assays with different Sudan glucuronides and X-gluc demonstrated that the SudanIV variant is a valuable glucuronide substrate for the precise histochemical localization of GUS activity in transgenic plants.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Van der Eycken E,Terryn N,Goeman JL,Carlens G,Nerinckx W,Claeyssens M,Van der Eycken J,Van Montagu M,Brito-Arias M,Engler Gdoi
10.1007/s002990000219subject
Has Abstractpub_date
2000-10-01 00:00:00pages
966-970issue
10eissn
0721-7714issn
1432-203Xpii
10.1007/s002990000219journal_volume
19pub_type
杂志文章abstract::Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050321
更新日期:1997-09-01 00:00:00
abstract::Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232404
更新日期:1990-11-01 00:00:00
abstract:KEY MESSAGE:Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1331-z
更新日期:2012-12-01 00:00:00
abstract::Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00267652
更新日期:1981-08-01 00:00:00
abstract::Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-016-2017-8
更新日期:2016-09-01 00:00:00
abstract::Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193155
更新日期:1991-09-01 00:00:00
abstract:KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1739-3
更新日期:2015-05-01 00:00:00
abstract:: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000234
更新日期:2000-11-01 00:00:00
abstract::Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-010-0898-5
更新日期:2010-10-01 00:00:00
abstract:KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1705-5
更新日期:2015-02-01 00:00:00
abstract::Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272107
更新日期:1990-05-01 00:00:00
abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0230-6
更新日期:2007-03-01 00:00:00
abstract:KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1422-5
更新日期:2013-08-01 00:00:00
abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232265
更新日期:1990-12-01 00:00:00
abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0766-3
更新日期:2009-11-01 00:00:00
abstract::Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232527
更新日期:1992-05-01 00:00:00
abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0809-8
更新日期:2004-10-01 00:00:00
abstract::The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0534-9
更新日期:2008-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
更新日期:2005-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233096
更新日期:1992-10-01 00:00:00
abstract:KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1621-8
更新日期:2014-08-01 00:00:00
abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0254-y
更新日期:2007-03-01 00:00:00
abstract::Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000198
更新日期:2000-09-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
abstract:KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-021-02666-9
更新日期:2021-02-01 00:00:00
abstract::Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0657-z
更新日期:2009-03-01 00:00:00
abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2176-2
更新日期:2017-10-01 00:00:00
abstract:KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2228-7
更新日期:2018-02-01 00:00:00
abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232028
更新日期:1995-02-01 00:00:00
abstract::A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237054
更新日期:1993-02-01 00:00:00