Sudan-β-d-glucuronides and their use for the histochemical localization of β-glucuronidase activity in transgenic plants.

Abstract:

: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used for histochemical localization of β-glucuronidase (GUS) activity in transgenic plants (Petunia hybrida, Arabidopsis thaliana, and Nicotiana tabacum) that contained the GUS reporter system. Because the cleavage of the β-glucuronide results in the liberation of an insoluble Sudan dye, Sudan substrates gave no diffusion artifacts as described for the commonly used 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-gluc). A comparison of assays with different Sudan glucuronides and X-gluc demonstrated that the SudanIV variant is a valuable glucuronide substrate for the precise histochemical localization of GUS activity in transgenic plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Van der Eycken E,Terryn N,Goeman JL,Carlens G,Nerinckx W,Claeyssens M,Van der Eycken J,Van Montagu M,Brito-Arias M,Engler G

doi

10.1007/s002990000219

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

966-970

issue

10

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990000219

journal_volume

19

pub_type

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