Abstract：
:A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Average transformation frequencies ranged from 2.0 to 15.5% depending on the construct, genotype and type of tissue used for transformation. The highest transformation rate was obtained for hypocotyl explants from tomato variety SG048. The ploidy levels of 264 independent transgenic events and 233 non-transgenic plants regenerated from tissue culture were assessed by flow cytometry. The incidence of polyploids within the total population of transgenic plants varied from 10 to 78% and was not significantly different from the non-transgenic population. The greatest variation in the proportion of polyploids was observed in plants derived from different explant types, both in transgenic and non-transgenic regenerants, across three studied genotypes. Transgenic and non-transgenic plants regenerated from leaves included the highest number of normal diploid plants (82-100%), followed by cotyledon petiole-derived plants (63-78%). Transgenic plants produced from hypocotyls contained 22-58% diploids depending on the genotype used in transformation. Results described in this study demonstrate that, although transformation frequencies for leaf tissue are still lower under current protocols, the high percentage of diploids obtained make leaf tissue an attractive transformation target.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Sigareva M,Spivey R,Willits MG,Kramer CM,Chang YFdoi
10.1007/s00299-004-0809-8keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
236-45issue
4eissn
0721-7714issn
1432-203Xjournal_volume
23pub_type
杂志文章abstract：:Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0550-0
更新日期：2003-02-01 00:00:00
abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232946
更新日期：1994-06-01 00:00:00
abstract：:Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1735-z
更新日期：2015-04-01 00:00:00
abstract：:Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239891
更新日期：1994-01-01 00:00:00
abstract：KEY MESSAGE:We report global gene expression patterns of poplar in response to chilling stress. A total of 1,085 significantly differentially expressed genes, involved in photosynthesis, signal transduction, and regulation of transcription, were identified. To understand the gene network underlying the response to chil...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1454-x
更新日期：2013-09-01 00:00:00
abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233065
更新日期：1993-08-01 00:00:00
abstract：:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01275445
更新日期：1996-11-01 00:00:00
abstract：:The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269212
更新日期：1985-04-01 00:00:00
abstract：KEY MESSAGE:Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02634-9
更新日期：2021-02-01 00:00:00
abstract：:Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-016-2017-8
更新日期：2016-09-01 00:00:00
abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237431
更新日期：1993-04-01 00:00:00
abstract：:The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0444-2
更新日期：2008-01-01 00:00:00
abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1895-5
更新日期：2016-02-01 00:00:00
abstract：:The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-007-0390-z
更新日期：2007-09-01 00:00:00
abstract：KEY MESSAGE:SbNAC genes (131) encoding 183 proteins were identified from the sorghum genome and characterized. The expression patterns of SbSNACs were evaluated at three sampling time points under post-flowering drought stress. NAC proteins are specific transcription factors in plants, playing vital roles in developmen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02371-8
更新日期：2019-03-01 00:00:00
abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233813
更新日期：1991-11-01 00:00:00
abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269555
更新日期：1987-10-01 00:00:00
abstract：:The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0753-z
更新日期：2004-05-01 00:00:00
abstract：:An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890875
更新日期：1996-12-01 00:00:00
abstract：:Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00276666
更新日期：1987-04-01 00:00:00
abstract：:An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233776
更新日期：1994-12-01 00:00:00
abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0534-9
更新日期：2008-07-01 00:00:00
abstract：:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272536
更新日期：1988-06-01 00:00:00
abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1164-1
更新日期：2012-02-01 00:00:00
abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-010-0898-5
更新日期：2010-10-01 00:00:00
abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235072
更新日期：1992-06-01 00:00:00
abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050753
更新日期：2000-04-01 00:00:00
abstract：: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050702
更新日期：1999-11-01 00:00:00
abstract：:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269296
更新日期：1984-12-01 00:00:00
abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235258
更新日期：1992-03-01 00:00:00
