Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine.

Abstract:

:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Guri A,Zelcer A,Izhar S

doi

10.1007/BF00269296

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

219-21

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

3

pub_type

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