In vitro culture of isolated microspores and regeneration of plants in Brassica campestris.

abstract：:In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain A(w) in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binar...

journal_title：Plant cell reports

authors： Figueiredo JF,Römer P,Lahaye T,Graham JH,White FF,Jones JB

更新日期：2011-07-01 00:00:00

abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...

journal_title：Plant cell reports

authors： Marigo G,Belkoura M

更新日期：1985-12-01 00:00:00

abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...

journal_title：Plant cell reports

authors： Li LC,Kohlenbach HW

更新日期：1982-10-01 00:00:00

abstract：:Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...

journal_title：Plant cell reports

authors： Lee-Parsons CW,Royce AJ

更新日期：2006-06-01 00:00:00

abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...

journal_title：Plant cell reports

authors： Dmitriev A,Djatsok J,Grodzinsky D

更新日期：1996-09-01 00:00:00

abstract：:Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...

journal_title：Plant cell reports

authors： Xu J,Chai T,Zhang Y,Lang M,Han L

更新日期：2009-08-01 00:00:00

abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...

journal_title：Plant cell reports

authors： Feng S,Tan J,Zhang Y,Liang S,Xiang S,Wang H,Chai T

更新日期：2017-02-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...

journal_title：Plant cell reports

authors： Bohorova NE,Cocking EC,Power JB

更新日期：1986-08-01 00:00:00

abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...

journal_title：Plant cell reports

authors： Manzocchi LA,Bianchi MW,Viotti A

更新日期：1989-03-01 00:00:00

abstract：:The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...

journal_title：Plant cell reports

authors： Redha A,Attia T,Büter B,Saisingtong S,Stamp P,Schmid JE

更新日期：1998-09-01 00:00:00

abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

journal_title：Plant cell reports

authors： Hu Y,Sun G

更新日期：2010-10-01 00:00:00

abstract：:Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

journal_title：Plant cell reports

authors： Smith MK,McComb JA

更新日期：1983-06-01 00:00:00

abstract：:Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...

journal_title：Plant cell reports

authors： Ellis JS,Jennings AC,Edwards LA,Mavandad M,Lamb CJ,Dixon RA

更新日期：1989-12-01 00:00:00

abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...

journal_title：Plant cell reports

authors： Utomo HS,Croughan SS,Croughan TP

更新日期：1995-01-01 00:00:00

abstract：:Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to dir...

journal_title：Plant cell reports

authors： Guang-Min X,Zhongyi L,Guang-Qin G,Hui-Min C

更新日期：1992-04-01 00:00:00

abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...

journal_title：Plant cell reports

authors： Gao J,Lee JM,An G

更新日期：1991-12-01 00:00:00

abstract：KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...

journal_title：Plant cell reports

authors： Ma X,Zhang J,Han B,Tang J,Cui D,Han L

更新日期：2019-03-01 00:00:00

abstract：:Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

journal_title：Plant cell reports

authors： Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

更新日期：2010-08-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00

abstract：:Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...

journal_title：Plant cell reports

authors： Chen WH,Hsu CY,Cheng HY,Chang H,Chen HH,Ger MJ

更新日期：2011-06-01 00:00:00

abstract：:Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...

journal_title：Plant cell reports

authors： Stöcker S,Guitton MC,Barth A,Mühlbach HP

更新日期：1993-09-01 00:00:00

abstract：:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...

journal_title：Plant cell reports

authors： Davis DG,Olson PA,Stolzenberg RL

更新日期：1988-06-01 00:00:00

abstract：:Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...

journal_title：Plant cell reports

authors： Lees GL

更新日期：1988-05-01 00:00:00

abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...

journal_title：Plant cell reports

authors： Kokko HI,Kärenlampi SO

更新日期：1998-07-01 00:00:00

abstract：UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...

journal_title：Plant cell reports

authors： Chen L,Chen Y,Jiang J,Chen S,Chen F,Guan Z,Fang W

更新日期：2012-09-01 00:00:00

abstract：:Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...

journal_title：Plant cell reports

authors： Burnett L,Yarrow S,Huang B

更新日期：1992-05-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...

journal_title：Plant cell reports

authors： Minocha R,Minocha SC,Komamine A,Shortle WC

更新日期：1991-06-01 00:00:00