Abstract:
:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. They developed to mature embryos when the auxin was reduced or omitted.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Li LC,Kohlenbach HWdoi
10.1007/BF00270237subject
Has Abstractpub_date
1982-10-01 00:00:00pages
209-11issue
5eissn
0721-7714issn
1432-203Xjournal_volume
1pub_type
杂志文章abstract::Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1688-2
更新日期:2015-01-01 00:00:00
abstract::Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269528
更新日期:1988-10-01 00:00:00
abstract::Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716668
更新日期:1989-06-01 00:00:00
abstract:KEY MESSAGE:Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02502-1
更新日期:2020-04-01 00:00:00
abstract::In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0988-4
更新日期:2011-05-01 00:00:00
abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
abstract:KEY MESSAGE:When fused to " Pr AlSAP " promoter, transcripts of gusA exhibited similar accumulation patterns in transgenic rice as AlSAP transcripts in A. littoralis. Pr AlSAP can be used for engineering abiotic stress tolerance. We previously showed that ectopic expression of a stress-associated protein gene from Aelu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1825-6
更新日期:2015-10-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract::Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232127
更新日期:1990-06-01 00:00:00
abstract:KEY MESSAGE:Transcriptome analysis of barley embryogenic callus from isolated microspore culture under salt stress uncovered a role of translation inhibition and selective activation of stress-specific proteins in cellular defense. Soil salinity is one of the major abiotic stresses which constrains the plant growth and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1986-y
更新日期:2016-08-01 00:00:00
abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1702-8
更新日期:2015-02-01 00:00:00
abstract::A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050470
更新日期:1998-06-01 00:00:00
abstract::An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effect...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269273
更新日期:1981-12-01 00:00:00
abstract::Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium and by temperature. The highest concentration of essential oils was obtained when regenerated flowers were...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231941
更新日期:1995-06-01 00:00:00
abstract::A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237054
更新日期:1993-02-01 00:00:00
abstract::Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0695-1
更新日期:2009-06-01 00:00:00
abstract::LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000237
更新日期:2000-11-01 00:00:00
abstract:KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1726-0
更新日期:2015-03-01 00:00:00
abstract::In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. S...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232991
更新日期:1996-03-01 00:00:00
abstract:KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02543-x
更新日期:2020-08-01 00:00:00
abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0736-0
更新日期:2004-03-01 00:00:00
abstract::Chloroplast-nucleus communication takes place via processes called anterograde and retrograde signaling pathways. Discovery of the retrograde signaling pathways from the chloroplasts to the nucleus also raised an intriguing proposition that chloroplasts may serve as environmental sensors since multitudes of environmen...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02383-4
更新日期:2019-07-01 00:00:00
abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0697-3
更新日期:2004-01-01 00:00:00
abstract:: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050763
更新日期:2000-04-01 00:00:00
abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050303
更新日期:1997-07-01 00:00:00
abstract::Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272982
更新日期:1988-01-01 00:00:00
abstract:: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000276
更新日期:2000-12-01 00:00:00
abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269287
更新日期:1985-08-01 00:00:00