Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.

Abstract:

:In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Dillen W,Dijkstra I,Oud J

doi

10.1007/BF00232991

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

545-8

issue

7

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

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