Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.).

abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

journal_title：Plant cell reports

authors： Evans DA,Gamborg OL

更新日期：1982-04-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

journal_title：Plant cell reports

authors： Moore PJ,Moore AJ,Collins GB

更新日期：1994-07-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00

abstract：:Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...

journal_title：Plant cell reports

authors： Li X,Qin JC,Wang QY,Wu X,Lang CY,Pan HY,Gruber MY,Gao MJ

更新日期：2011-08-01 00:00:00

abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

journal_title：Plant cell reports

authors： Castillo AM,Cistué L

更新日期：1993-01-01 00:00:00

abstract：:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

journal_title：Plant cell reports

authors： Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

更新日期：1990-08-01 00:00:00

abstract：:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1982-06-01 00:00:00

abstract：:The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine...

journal_title：Plant cell reports

authors： Chi GL,Lin WS,Lee JE,Pua EC

更新日期：1994-03-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

journal_title：Plant cell reports

authors： Guo B,Gao M,Liu CZ

更新日期：2007-03-01 00:00:00

abstract：:A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...

journal_title：Plant cell reports

authors： Sahoo Y,Chand PK

更新日期：1998-12-01 00:00:00

abstract：KEY MESSAGE:Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled ...

journal_title：Plant cell reports

authors： Ayabe H,Kawai N,Shibamura M,Fukao Y,Fujimoto M,Tsutsumi N,Arimura SI

更新日期：2021-02-01 00:00:00

abstract：KEY MESSAGE:Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis. ABSTRACT:Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia ...

journal_title：Plant cell reports

authors： Yu Y,Ye W,He L,Cai X,Liu T,Liu J

更新日期：2013-11-01 00:00:00

abstract：:KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...

journal_title：Plant cell reports

authors： Terashima M,Fukukita A,Kodama R,Miki H,Suzuki M,Ikegami M,Tamura N,Yasuda A,Morikawa M,Matsumura S

更新日期：2013-03-01 00:00:00

abstract：:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...

journal_title：Plant cell reports

authors： Bassene JB,Froelicher Y,Navarro L,Ollitrault P,Ancillo G

更新日期：2011-06-01 00:00:00

abstract：:A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

journal_title：Plant cell reports

authors： Ayabe M,Sumi S

更新日期：1998-07-01 00:00:00

abstract：:Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...

journal_title：Plant cell reports

authors： Amarasinghe V,Dhami R,Carlson JE

更新日期：1996-03-01 00:00:00

abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...

journal_title：Plant cell reports

authors： Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono H

更新日期：2016-03-01 00:00:00

abstract：KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...

journal_title：Plant cell reports

authors： Häkkinen ST,Nygren H,Nohynek L,Puupponen-Pimiä R,Heiniö RL,Maiorova N,Rischer H,Ritala A

更新日期：2020-12-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...

journal_title：Plant cell reports

authors： Morales-Cedillo F,González-Solís A,Gutiérrez-Angoa L,Cano-Ramírez DL,Gavilanes-Ruiz M

更新日期：2015-04-01 00:00:00

abstract：: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...

journal_title：Plant cell reports

authors： Kästner U,Klahr A,Keller ERJ,Kahane R

更新日期：2001-02-01 00:00:00

abstract：:Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...

journal_title：Plant cell reports

authors： Iwai S,Kishi C,Nakata K,Kawashima N

更新日期：1986-10-01 00:00:00

abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...

journal_title：Plant cell reports

authors： Hasan MM,Kim HS,Jeon JH,Kim SH,Moon B,Song JY,Shim SH,Baek KH

更新日期：2014-06-01 00:00:00

abstract：:We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...

journal_title：Plant cell reports

authors： Tamilselvi D,Anand G,Swarup S

更新日期：2004-08-01 00:00:00

abstract：:Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....

journal_title：Plant cell reports

authors： Kavitha PG,Thomas G

更新日期：2008-11-01 00:00:00

abstract：:The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...

journal_title：Plant cell reports

authors： Kim MK,Lee BS,In JG,Sun H,Yoon JH,Yang DC

更新日期：2006-06-01 00:00:00

abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

journal_title：Plant cell reports

authors： Schumacher HM,Gundlach H,Fiedler F,Zenk MH

更新日期：1987-12-01 00:00:00