Elicitation of benzophenanthridine alkaloid synthesis in Eschscholtzia cell cultures.

Abstract:

:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora megasperma elicitor are inactive. The alkaloid synthesis is, however, strongly induced by certain polypeptide antibiotics. Out of 190 tested plant species the yeast elicitor provoked benzophenanthridine synthesis in 13 cultures. One of the branch point enzymes, namely the berberine bridge enzyme, catalysing the formation of (S)-scoulerine from (S)-reticuline, is strongly stimulated during the elicitation process. These results clearly demonstrate the induction of the benzophenanthridine biosynthetic pathway by microbial elicitors.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Schumacher HM,Gundlach H,Fiedler F,Zenk MH

doi

10.1007/BF00272770

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

410-3

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

6

pub_type

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