Abstract:
:Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro is first reported. In total, approximately 200 pieces (8 g) of embryogenic calluses were infected with the super-virulent A. tumefaciens strain EHA105 harboring the plant transformation plasmid pBI121/ricchi11 that contains the rice chitinase gene ricchi11. The presence and expression of the transgene ricchi11 in six independent transgenic lines was confirmed using polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR). Southern blot analysis of the six independent lines indicated that three out of six (50%) had integrated a single copy of the transgene, and the other three lines had two or three copies of the transgene. Compared to the particle bombardment transformation of taro method, which was used in the previous studies, the Agrobacterium-mediated transformation method obtained 43-fold higher transformation efficiency. In addition, these six transgenic lines via Agrobacterium may be more effective for transgene expression as a result of single-copy or low-copy insertion of the transgene than the single line with multiple copies of the transgene via particle bombardment. In a laboratory bioassay, all six transgenic lines exhibited increased tolerance to the fungal pathogen Sclerotium rolfsii, ranging from 42 to 63% reduction in lesion expansion.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
He X,Miyasaka SC,Fitch MM,Moore PH,Zhu YJdoi
10.1007/s00299-008-0519-8subject
Has Abstractpub_date
2008-05-01 00:00:00pages
903-9issue
5eissn
0721-7714issn
1432-203Xjournal_volume
27pub_type
杂志文章abstract::Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0144-3
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0422-8
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2006-y
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269538
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pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236381
更新日期:1992-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1139-2
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00299-010-0875-z
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journal_title:Plant cell reports
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pub_type: 杂志文章,评审
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0999-1
更新日期:2011-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2004-0
更新日期:2016-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232265
更新日期:1990-12-01 00:00:00
abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050481
更新日期:1998-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269711
更新日期:1986-02-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00