Abstract:
KEY MESSAGE:Wheat MAPK gene TaMPK14 is N starvation response and is crucial in modulating plant low-N stress tolerance. Improving plant N use efficiency (NUE) contributes largely to the sustainable crop production worldwide. In this study, TaMPK14, a mitogen-activated protein kinase (MAPK) family gene in T. aestivum, was characterized for the role in mediating N starvation response. TaMPK14 harbors conserved domain/motifs specified by the plant MAPK proteins. In vitro assay for kinase activity of TaMPK14 validated its phosphorylation nature. TaMPK14 transcripts were upregulated in both roots and leaves under low-N treatment; moreover, the expression levels induced by N starvation were gradually restored following the N recovery progression. These results suggested transcriptional response of TaMPK14 upon the low-N stress. Compared with wild type (WT), the TaMPK14 overexpressing lines in N. tabacum displayed improved growth and N accumulation traits under deficient-N treatment, which indicated the crucial roles of the MAPK gene in mediating N starvation response. Additionally, the lines treated by N starvation were shown to be improved on cellular ROS homeostasis, displaying higher antioxidant enzymes (AE) activities and less ROS accumulative amount than WT. The transcripts of nitrate transporter gene NtNRT2.1 and those of AE genes NtSOD1, NtCAT1;2, and NtPOD4 were significantly upregulated in N-deprived TaMPK14 lines; overexpression of them conferred plants enhanced N uptake capacity and AE activities, respectively. Moreover, RNA-seq datasets generated from N-deprived transgenic lines contained numerous differential genes involving modulating various biological process, cellular component, and molecular function. Together, our investigation suggested that TaMPK14 improves plant N starvation response through transcriptional regulation of distinct NRT and AE genes as well as modulation of associated biological processes.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Shi M,Wang Z,Ma Z,Song W,Lu W,Xiao Kdoi
10.1007/s00299-020-02564-6subject
Has Abstractpub_date
2020-10-01 00:00:00pages
1285-1299issue
10eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-020-02564-6journal_volume
39pub_type
杂志文章abstract::A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233766
更新日期:1994-12-01 00:00:00
abstract::Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276676
更新日期:1987-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268603
更新日期:1986-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-007-0390-z
更新日期:2007-09-01 00:00:00
abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269126
更新日期:1986-06-01 00:00:00
abstract::Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778536
更新日期:1989-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231549
更新日期:1990-07-01 00:00:00
abstract::Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270237
更新日期:1982-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2184-2
更新日期:2017-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0974-x
更新日期:2011-04-01 00:00:00
abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1768-y
更新日期:2015-05-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2006-y
更新日期:2016-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1096-9
更新日期:2011-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1067-1
更新日期:2011-08-01 00:00:00
abstract::Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0848-1
更新日期:2004-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233301
更新日期:1994-11-01 00:00:00
abstract:: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050763
更新日期:2000-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272364
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269250
更新日期:1986-04-01 00:00:00
abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272039
更新日期:1989-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233367
更新日期:1992-07-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232920
更新日期:1995-10-01 00:00:00
abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050415
更新日期:1998-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
abstract::KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1368-z
更新日期:2013-03-01 00:00:00
abstract::In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0942-5
更新日期:2011-03-01 00:00:00