Inefficient expression of the DNA-binding domain of GAL4 in transgenic plants.

Abstract:

:The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-binding domain or the presence of GAL4 recognition sequences in the promoter region of the reporter genes. Despite high levels of GAL4 mRNA, no translation product was detectable in transgenic plants indicating that the failure to detect functional GAL4 in plants is due to the inefficiency of GAL4 mRNA translation in plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

doi

10.1007/BF00232920

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

773-6

issue

12

eissn

0721-7714

issn

1432-203X

journal_volume

14

pub_type

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