Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion.

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:5-Deoxy-(iso)flavonoids are biosynthesized from 6'-deoxychalcone (isoliquiritigenin). The coaction of a reductase with chalcone synthase (CHS) has been established in soybean cells to be responsible for the synthesis of 6'-deoxychalcone. Western blot analysis of crude extracts from cultured cells of Glycyrrhiza echina...

journal_title：Plant cell reports

authors： Haranô K,Okada N,Furuno T,Takahashi T,Ayabe S,Welle R

更新日期：1993-01-01 00:00:00

abstract：:An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...

journal_title：Plant cell reports

authors： Siril EA,Dhar U

更新日期：1997-06-01 00:00:00

abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...

journal_title：Plant cell reports

authors： Martins TF,Souza PFN,Alves MS,Silva FDA,Arantes MR,Vasconcelos IM,Oliveira JTA

更新日期：2020-08-01 00:00:00

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

journal_title：Plant cell reports

authors： Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

更新日期：2011-12-01 00:00:00

abstract：:Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...

journal_title：Plant cell reports

authors： Vuke TM,Mott RL

更新日期：1987-04-01 00:00:00

abstract：:A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

journal_title：Plant cell reports

authors： Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

更新日期：2005-05-01 00:00:00

abstract：:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...

journal_title：Plant cell reports

authors： Shirasawa-Seo N,Sano Y,Nakamura S,Murakami T,Gotoh Y,Naito Y,Hsia CN,Seo S,Mitsuhara I,Kosugi S,Ohashi Y

更新日期：2005-06-01 00:00:00

abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

journal_title：Plant cell reports

authors： Schmidt M,Parrott W

更新日期：2001-07-01 00:00:00

abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...

journal_title：Plant cell reports

authors： Utomo HS,Croughan SS,Croughan TP

更新日期：1995-01-01 00:00:00

abstract：:The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...

journal_title：Plant cell reports

authors： Kim MK,Lee BS,In JG,Sun H,Yoon JH,Yang DC

更新日期：2006-06-01 00:00:00

abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...

journal_title：Plant cell reports

authors： Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ES

更新日期：1997-09-01 00:00:00

abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

journal_title：Plant cell reports

authors： Schumacher HM,Gundlach H,Fiedler F,Zenk MH

更新日期：1987-12-01 00:00:00

abstract：KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...

journal_title：Plant cell reports

authors： Shi G,Guo X,Guo J,Liu L,Hua J

更新日期：2015-06-01 00:00:00

abstract：KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

journal_title：Plant cell reports

authors： Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

更新日期：2021-01-01 00:00:00

abstract：KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...

journal_title：Plant cell reports

authors： Honma Y,Yamakawa T

更新日期：2019-11-01 00:00:00

abstract：:Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...

journal_title：Plant cell reports

authors： Vergara R,Verde F,Pitto L,Lo Schiavo F,Terzi M

更新日期：1990-05-01 00:00:00

abstract：:Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...

journal_title：Plant cell reports

authors： Rock CD,Quatrano RS

更新日期：1996-01-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...

journal_title：Plant cell reports

authors： Yoshihara T,Hodoshima H,Miyano Y,Shoji K,Shimada H,Goto F

更新日期：2006-04-01 00:00:00

abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...

journal_title：Plant cell reports

authors： Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

更新日期：2000-09-01 00:00:00

abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

journal_title：Plant cell reports

authors： Murphy JF,Kyle MM

更新日期：1994-04-01 00:00:00

abstract：KEY MESSAGE:Transcription factors from mammals and plants, which play a role in innate immunity, interact with the same microbe-associated molecular pattern (MAMP)-responsive sequences from Arabidopsis thaliana. The interaction of mouse NF-κB p65 with MAMP-responsive sequences containing the core motif GACTTT of the WT...

journal_title：Plant cell reports

authors： Kanofsky K,Riggers J,Staar M,Strauch CJ,Arndt LC,Hehl R

更新日期：2019-09-01 00:00:00

abstract：KEY MESSAGE:Two lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system. Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl ...

journal_title：Plant cell reports

authors： Sykłowska-Baranek K,Rymaszewski W,Gaweł M,Rokicki P,Pilarek M,Grech-Baran M,Hennig J,Pietrosiuk A

更新日期：2019-01-01 00:00:00

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manip...

journal_title：Plant cell reports

authors： Ahuja PS,Hadiuzzaman S,Davey MR,Cocking EC

更新日期：1983-04-01 00:00:00

abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

journal_title：Plant cell reports

authors： Hosoyama H,Irie K,Abe K,Arai S

更新日期：1995-12-01 00:00:00

abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...

journal_title：Plant cell reports

authors： Marigo G,Belkoura M

更新日期：1985-12-01 00:00:00

abstract：:The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...

journal_title：Plant cell reports

authors： Bean SJ,Gooding PS,Mullincaux PM,Davies DR

更新日期：1997-05-01 00:00:00