Callus production from leaf protoplasts of various cultivars of bean (Phaseolus vulgaris L.).

abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...

journal_title：Plant cell reports

authors： McKenzie MJ,Mett V,Jameson PE

更新日期：2000-01-01 00:00:00

abstract：:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1982-06-01 00:00:00

abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

journal_title：Plant cell reports

authors： Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

更新日期：2015-02-01 00:00:00

abstract：:Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...

journal_title：Plant cell reports

authors： Fári M,Nagy I,Csányi M,Mitykó J,Andrásfalvy A

更新日期：1995-01-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) activity, contents of phenolic acids and ethylene production during the lag-phase, and contents of phenolic acids at the late exponential phase, showed significant differences in embryogenic (EC) and non-embryogenic (NEC) suspension cultures of Medicago sativa L. Maximum PAL activity ...

journal_title：Plant cell reports

authors： Cvikrová M,Meravý L,Macháčková I,Eder J

更新日期：1991-08-01 00:00:00

abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...

journal_title：Plant cell reports

authors： Zhong H,Srinivasan C,Sticklen MB

更新日期：1991-11-01 00:00:00

abstract：:Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...

journal_title：Plant cell reports

authors： Towill LE,Jarret RL

更新日期：1992-05-01 00:00:00

abstract：KEY MESSAGE:Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis. ABSTRACT:Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia ...

journal_title：Plant cell reports

authors： Yu Y,Ye W,He L,Cai X,Liu T,Liu J

更新日期：2013-11-01 00:00:00

abstract：:Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...

journal_title：Plant cell reports

authors： Quiros CF,McGrath M,Stites JL

更新日期：1987-04-01 00:00:00

abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...

journal_title：Plant cell reports

authors： Hoshino Y,Nishino E,Mii M

更新日期：2000-04-01 00:00:00

abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...

journal_title：Plant cell reports

authors： Tisserat B,Galletta PD

更新日期：1993-11-01 00:00:00

abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...

journal_title：Plant cell reports

authors： Martins TF,Souza PFN,Alves MS,Silva FDA,Arantes MR,Vasconcelos IM,Oliveira JTA

更新日期：2020-08-01 00:00:00

abstract：:The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...

journal_title：Plant cell reports

authors： de Abreu IS,Carvalho CR,Clarindo WR

更新日期：2008-07-01 00:00:00

abstract：:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...

journal_title：Plant cell reports

authors： Nakagawa K,Konagai A,Fukui H,Tabata M

更新日期：1984-12-01 00:00:00

abstract：KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...

journal_title：Plant cell reports

authors： Pérez L,Soto E,Farré G,Juanos J,Villorbina G,Bassie L,Medina V,Serrato AJ,Sahrawy M,Rojas JA,Romagosa I,Muñoz P,Zhu C,Christou P

更新日期：2019-03-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...

journal_title：Plant cell reports

authors： Rosnow J,Offermann S,Park J,Okita TW,Tarlyn N,Dhingra A,Edwards GE

更新日期：2011-08-01 00:00:00

abstract：:Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial r...

journal_title：Plant cell reports

authors： Luo C,Guo C,Wang W,Wang L,Chen L

更新日期：2014-02-01 00:00:00

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：:High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...

journal_title：Plant cell reports

authors： Pattnaik SK,Chand PK

更新日期：1997-04-01 00:00:00

abstract：: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...

journal_title：Plant cell reports

authors： Xie DY,Hong Y

更新日期：2001-01-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...

journal_title：Plant cell reports

authors： Reiner T,Hoefle C,Huesmann C,Ménesi D,Fehér A,Hückelhoven R

更新日期：2015-03-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...

journal_title：Plant cell reports

authors： Hily JM,Liu Z

更新日期：2009-03-01 00:00:00

abstract：:Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...

journal_title：Plant cell reports

authors： Vergara R,Verde F,Pitto L,Lo Schiavo F,Terzi M

更新日期：1990-05-01 00:00:00

abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

journal_title：Plant cell reports

authors： Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

更新日期：2005-08-01 00:00:00

abstract：KEY MESSAGE:Candidate genes were identified and the role of phytohormones such as JA-Me and ABA in the synthesis of S-RNase was emphasized in pear self-incompatibility. Self-incompatibility (SI) occurs widely in flowering plants as an intraspecific reproductive barrier. This phenomenon promotes variation within species...

journal_title：Plant cell reports

authors： Shi D,Tang C,Wang R,Gu C,Wu X,Hu S,Jiao J,Zhang S

更新日期：2017-11-01 00:00:00

abstract：:An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

journal_title：Plant cell reports

authors： Esteves P,Belzile F

更新日期：2014-06-01 00:00:00

abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

journal_title：Plant cell reports

authors： Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

更新日期：2003-03-01 00:00:00

abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

journal_title：Plant cell reports

authors： Schmidt M,Parrott W

更新日期：2001-07-01 00:00:00