Agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. 'Kecskeméti lila').

Abstract:

:Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. Transformants were recovered from embryogenic calli induced by 4 mg/lα-naphthaleneacetic acid (NAA), and from organogenic calli induced by the addition of 2 mg/l zeatin plus 0.01 mg/l NAA. Nineteen independent transgenic lines were grown to maturity. The structural integrity, expression and sexual transmission of the introduced genes for neomycin phosphotransferase and ß-glucuronidase (gus) were investigated.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Fári M,Nagy I,Csányi M,Mitykó J,Andrásfalvy A

doi

10.1007/BF01690259

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

82-6

issue

1-2

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

杂志文章
  • Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment.

    abstract:: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050004

    authors: Becker DK,Dugdale B,Smith MK,Harding RM,Dale JL

    更新日期:2000-01-01 00:00:00

  • Length of the dark period affects flower opening and the expression of circadian-clock associated genes as well as xyloglucan endotransglucosylase/hydrolase genes in petals of morning glory (Ipomoea nil).

    abstract:KEY MESSAGE:We isolated differentially expressed and dark-responsive genes during flower development and opening in petals of morning glory. Flower opening usually depends on petal expansion and is regulated by both genetic and environmental factors. Flower opening in morning glory (Ipomoea nil) is controlled by the da...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1601-z

    authors: Shinozaki Y,Tanaka R,Ono H,Ogiwara I,Kanekatsu M,van Doorn WG,Yamada T

    更新日期:2014-07-01 00:00:00

  • Endogenous jasmonic and salicylic acids levels in the Cd-hyperaccumulator Noccaea (Thlaspi) praecox exposed to fungal infection and/or mechanical stress.

    abstract:KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-013-1427-0

    authors: Llugany M,Martin SR,Barceló J,Poschenrieder C

    更新日期:2013-08-01 00:00:00

  • Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

    abstract::An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00238594

    authors: Watad AA,Kochba M,Nissim A,Gaba V

    更新日期:1995-03-01 00:00:00

  • Micropropagation of mature Chinese tallow tree (Sapium sebiferum Roxb.).

    abstract::An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01275506

    authors: Siril EA,Dhar U

    更新日期:1997-06-01 00:00:00

  • Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana.

    abstract:KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2356-8

    authors: Razzak MA,Lee J,Lee DW,Kim JH,Yoon HS,Hwang I

    更新日期:2019-02-01 00:00:00

  • Characterization of promoter of EgPAL1, a novel PAL gene from the oil palm Elaeis guineensis Jacq.

    abstract:KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2228-7

    authors: Yusuf CYL,Abdullah JO,Shaharuddin NA,Abu Seman I,Abdullah MP

    更新日期:2018-02-01 00:00:00

  • Genotype-specific heat shock proteins in two maize inbreds.

    abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233096

    authors: Jorgensen JA,Weng J,Ho TH,Nguyen HT

    更新日期:1992-10-01 00:00:00

  • The Arabidopsis thaliana hypocotyl, a model to identify and study control mechanisms of cellular expansion.

    abstract::Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-014-1591-x

    authors: Boron AK,Vissenberg K

    更新日期:2014-05-01 00:00:00

  • Introduction of a chimeric gene encoding an oryzacystatin-β-glucuronidase fusion protein into rice protoplasts and regeneration of transformed plants.

    abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193714

    authors: Hosoyama H,Irie K,Abe K,Arai S

    更新日期:1995-12-01 00:00:00

  • Growth of loblolly pine callus on a variety of carbohydrate sources.

    abstract::Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00276676

    authors: Vuke TM,Mott RL

    更新日期:1987-04-01 00:00:00

  • Improving the efficiency of isolated microspore culture in six-row spring barley: I-optimization of key physical factors.

    abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1583-x

    authors: Esteves P,Belzile F

    更新日期:2014-06-01 00:00:00

  • Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

    abstract::Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234579

    authors: Warkentin TD,McHughen A

    更新日期:1991-12-01 00:00:00

  • The Arabidopsis CORI3 promoter contains two cis-acting regulatory regions required for transcriptional activity in companion cells.

    abstract::Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1080-4

    authors: Tsuwamoto R,Harada T

    更新日期:2011-09-01 00:00:00

  • Metabolic engineering of isoflavone genistein in Brassica napus with soybean isoflavone synthase.

    abstract::Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1052-8

    authors: Li X,Qin JC,Wang QY,Wu X,Lang CY,Pan HY,Gruber MY,Gao MJ

    更新日期:2011-08-01 00:00:00

  • Efficient callus formation and plant regeneration from leaves of oats (Avena sativa L.).

    abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00238604

    authors: Chen H,Xu G,Loschke DC,Tomaska L,Rolfe BG

    更新日期:1995-03-01 00:00:00

  • Introduction of foreign genes into Pharbitis nil calli using a vector derived from Agrobacterium pTi.

    abstract::Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00274124

    authors: Araki T,Hirano H,Naito S,Komeda Y

    更新日期:1989-05-01 00:00:00

  • Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.

    abstract::The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232364

    authors: Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

    更新日期:1996-01-01 00:00:00

  • Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees.

    abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0604-y

    authors: Hernández I,Celestino C,Alegre J,Toribio M

    更新日期:2003-04-01 00:00:00

  • Quantitative detection of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) by real-time polymerase chain reaction.

    abstract::Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990100326

    authors: Schmidt M,Parrott W

    更新日期:2001-07-01 00:00:00

  • Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture.

    abstract::Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-012-1320-2

    authors: Krzewska M,Czyczyło-Mysza I,Dubas E,Gołębiowska-Pikania G,Golemiec E,Stojałowski S,Chrupek M,Zur I

    更新日期:2012-11-01 00:00:00

  • Screening of diploid Medicago sativa germplasm for somatic embryogenesis.

    abstract::Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a hi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269379

    authors: Meijer EG,Brown DC

    更新日期:1985-10-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232275

    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Stable transformation of papaya via microprojectile bombardment.

    abstract::Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232177

    authors: Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

    更新日期:1990-08-01 00:00:00

  • Expression of the green fluorescent protein gene in conifer tissues.

    abstract::The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01088278

    authors: Tian L,Séguin A,Charest PJ

    更新日期:1997-02-01 00:00:00

  • Transgenic turf-type tall fescue (Festuca amndinacea Schreb.) plants regenerated from protoplasts.

    abstract::To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00236381

    authors: Ha SB,Wu FS,Thorne TK

    更新日期:1992-11-01 00:00:00

  • Effects of drought-re-watering-drought on the photosynthesis physiology and secondary metabolite production of Bupleurum chinense DC.

    abstract:KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02436-8

    authors: Yang L,Zhao Y,Zhang Q,Cheng L,Han M,Ren Y,Yang L

    更新日期:2019-09-01 00:00:00

  • Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro.

    abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232608

    authors: Kwa SH,Wee YC,Loh CS

    更新日期:1991-10-01 00:00:00

  • Stable endosperm cell suspension cultures from wild-type and opaque-2 maize.

    abstract::Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232330

    authors: Manzocchi LA

    更新日期:1991-02-01 00:00:00

  • Ultrastructure and histochemical analysis of extracellular matrix surface network in kiwifruit endosperm-derived callus culture.

    abstract::The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0534-9

    authors: Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Swierczyńska J,Góralski G,Slesak H,Bohdanowicz J

    更新日期:2008-07-01 00:00:00