Abstract：
:The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires a great quantity of intact metaphasic chromosomes and methods that allow the characterization of individual chromosomes; however, the obtainment of adequate number of metaphases can be difficult in some species like C. annuum. In order to estimate the DNA content of each C. annuum var. annuum cv. "New Mexican" chromosome, flow and image cytometries were associated with the cytogenetic methodology. First, the DNA amount (2C = 6.90 pg) was established by flow cytometry. Integrated optical density (IOD) values were calculated by image cytometry for each Feulgen stained metaphasic chromosome. Then, by distributing the correspondent metaphasic value (4C = 13.80 pg) proportionally to average IOD values, the following chromosomal DNA contents were obtained in pg: 0.74 (chromosome 1), 0.67 (2), 0.61 (3, 4), 0.60 (5), 0.59 (6, 7), 0.58 (8), 0.57 (9), 0.56 (10) and 0.39 (11, 12). This study reports an alternative and reproducible technique that makes quantifying the chromosomal DNA content possible.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
de Abreu IS,Carvalho CR,Clarindo WRdoi
10.1007/s00299-008-0539-4subject
Has Abstractpub_date
2008-07-01 00:00:00pages
1227-33issue
7eissn
0721-7714issn
1432-203Xjournal_volume
27pub_type
杂志文章abstract：:Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01690259
更新日期：1995-01-01 00:00:00
abstract：:Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232275
更新日期：1990-12-01 00:00:00
abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1895-5
更新日期：2016-02-01 00:00:00
abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0695-1
更新日期：2009-06-01 00:00:00
abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232378
更新日期：1992-01-01 00:00:00
abstract：KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2144-x
更新日期：2017-07-01 00:00:00
abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000237
更新日期：2000-11-01 00:00:00
abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1568-9
更新日期：2014-06-01 00:00:00
abstract：:A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0736-0
更新日期：2004-03-01 00:00:00
abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0224-4
更新日期：2007-02-01 00:00:00
abstract：:Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0650-6
更新日期：2009-03-01 00:00:00
abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235082
更新日期：1992-06-01 00:00:00
abstract：:An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1583-x
更新日期：2014-06-01 00:00:00
abstract：:Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269240
更新日期：1986-04-01 00:00:00
abstract：:Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233315
更新日期：1994-02-01 00:00:00
abstract：KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2007-x
更新日期：2016-09-01 00:00:00
abstract：KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2088-6
更新日期：2017-02-01 00:00:00
abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050321
更新日期：1997-09-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1621-8
更新日期：2014-08-01 00:00:00
abstract：:Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01092764
更新日期：1997-04-01 00:00:00
abstract：:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0687-5
更新日期：2003-11-01 00:00:00
abstract：:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00274124
更新日期：1989-05-01 00:00:00
abstract：:A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0682-x
更新日期：2003-11-01 00:00:00
abstract：:Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0770-7
更新日期：2009-11-01 00:00:00
abstract：:Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268487
更新日期：1987-06-01 00:00:00
abstract：:An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233776
更新日期：1994-12-01 00:00:00
abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0499-0
更新日期：2008-04-01 00:00:00
abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232932
更新日期：1994-08-01 00:00:00
abstract：:The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269924
更新日期：1988-08-01 00:00:00
