Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets.

Abstract:

:Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Shoyama Y,Zhu XX,Nakai R,Shiraishi S,Kohda H

doi

10.1007/BF01092764

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

450-453

issue

7

eissn

0721-7714

issn

1432-203X

pii

10.1007/BF01092764

journal_volume

16

pub_type

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