Genetic transformation of Populus nigra by Agrobacterium tumefaciens.

abstract：:Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-01-01 00:00:00

abstract：:An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

journal_title：Plant cell reports

authors： Esteves P,Belzile F

更新日期：2014-06-01 00:00:00

abstract：:Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transforma...

journal_title：Plant cell reports

authors： Trypsteen M,Van Lijsebettens M,Van Severen R,Van Montagu M

更新日期：1991-06-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...

journal_title：Plant cell reports

authors： Bhatt D,Parrott WA,Collins GB,Hildebrand DF

更新日期：1991-03-01 00:00:00

abstract：:Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...

journal_title：Plant cell reports

authors： Ford KG

更新日期：1990-01-01 00:00:00

abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...

journal_title：Plant cell reports

authors： Sonia,Saini R,Singh RP,Jaiwal PK

更新日期：2007-02-01 00:00:00

abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

journal_title：Plant cell reports

authors： Rajasubramaniam S,Pardha Saradhi P

更新日期：1994-08-01 00:00:00

abstract：:Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...

journal_title：Plant cell reports

authors： Burnett L,Yarrow S,Huang B

更新日期：1992-05-01 00:00:00

abstract：:Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...

journal_title：Plant cell reports

authors： Villarreal ML,Rojas G

更新日期：1996-11-01 00:00:00

abstract：:Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

journal_title：Plant cell reports

authors： Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

更新日期：2008-06-01 00:00:00

abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

journal_title：Plant cell reports

authors： Wei W,Zhang Y,Han L,Guan Z,Chai T

更新日期：2008-04-01 00:00:00

abstract：:Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...

journal_title：Plant cell reports

authors： Hobbs SL,Jackson JA,Baliski DS,Delong CM,Mahon JD

更新日期：1990-06-01 00:00:00

abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...

journal_title：Plant cell reports

authors： Soares EL,Lima MLB,Nascimento JRS,Soares AA,Coutinho ÍAC,Campos FAP

更新日期：2017-11-01 00:00:00

abstract：KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...

journal_title：Plant cell reports

authors： Li YL,Yu YK,Zhu KM,Ding LN,Wang Z,Yang YH,Cao J,Xu LZ,Li YM,Tan XL

更新日期：2021-02-01 00:00:00

abstract：:The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...

journal_title：Plant cell reports

authors： Dixit A,Ma KH,Yu JW,Cho EG,Park YJ

更新日期：2006-03-01 00:00:00

abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...

journal_title：Plant cell reports

authors： Lutz KA,Martin C,Khairzada S,Maliga P

更新日期：2015-10-01 00:00:00

abstract：KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...

journal_title：Plant cell reports

authors： Gao Y,Liu H,Zhang K,Li F,Wu M,Xiang Y

更新日期：2021-01-01 00:00:00

abstract：:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...

journal_title：Plant cell reports

authors： Shim YS,Kasha KJ

更新日期：2003-07-01 00:00:00

abstract：KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...

journal_title：Plant cell reports

authors： Lv F,Li P,Zhang R,Li N,Guo W

更新日期：2016-04-01 00:00:00

abstract：:Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...

journal_title：Plant cell reports

authors： Manzocchi LA

更新日期：1991-02-01 00:00:00

abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...

journal_title：Plant cell reports

authors： Dmitriev A,Djatsok J,Grodzinsky D

更新日期：1996-09-01 00:00:00

abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...

journal_title：Plant cell reports

authors： McKenzie MJ,Mett V,Jameson PE

更新日期：2000-01-01 00:00:00

abstract：:Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...

journal_title：Plant cell reports

authors： Nayak P,Sen SK

更新日期：1989-05-01 00:00:00

abstract：:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

journal_title：Plant cell reports

authors： Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

更新日期：1990-08-01 00:00:00

abstract：:KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...

journal_title：Plant cell reports

authors： Terashima M,Fukukita A,Kodama R,Miki H,Suzuki M,Ikegami M,Tamura N,Yasuda A,Morikawa M,Matsumura S

更新日期：2013-03-01 00:00:00

abstract：:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

journal_title：Plant cell reports

authors： Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

更新日期：1997-03-01 00:00:00

abstract：:Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...

journal_title：Plant cell reports

authors： Yamada Y,Yang ZQ,Tang DT

更新日期：1986-04-01 00:00:00

abstract：:Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...

journal_title：Plant cell reports

authors： Sreenivasulu N

更新日期：2017-05-01 00:00:00

abstract：:We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...

journal_title：Plant cell reports

authors： van der Graaff E,Hooykaas PJ

更新日期：1996-04-01 00:00:00