Abstract:
:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi Tdoi
10.1007/BF01146782subject
Has Abstractpub_date
1997-03-01 00:00:00pages
401-405issue
6eissn
0721-7714issn
1432-203Xpii
10.1007/BF01146782journal_volume
16pub_type
杂志文章abstract:KEY MESSAGE:Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary element...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1379-9
更新日期:2013-04-01 00:00:00
abstract::Phenylalanine ammonia-lyase (PAL) activity, contents of phenolic acids and ethylene production during the lag-phase, and contents of phenolic acids at the late exponential phase, showed significant differences in embryogenic (EC) and non-embryogenic (NEC) suspension cultures of Medicago sativa L. Maximum PAL activity ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232569
更新日期:1991-08-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
abstract::To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232106
更新日期:1991-01-01 00:00:00
abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233055
更新日期:1993-08-01 00:00:00
abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571295
更新日期:1985-05-01 00:00:00
abstract::In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233638
更新日期:1995-01-01 00:00:00
abstract:KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02625-w
更新日期:2021-01-01 00:00:00
abstract::In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01088288
更新日期:1997-02-01 00:00:00
abstract::A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0499-0
更新日期:2008-04-01 00:00:00
abstract::Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00271999
更新日期:1987-07-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract::The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0835-7
更新日期:2010-05-01 00:00:00
abstract::A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237054
更新日期:1993-02-01 00:00:00
abstract::Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232981
更新日期:1996-03-01 00:00:00
abstract::Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-016-2017-8
更新日期:2016-09-01 00:00:00
abstract:KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2039-2
更新日期:2016-11-01 00:00:00
abstract::Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193155
更新日期:1991-09-01 00:00:00
abstract::Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...
journal_title:Plant cell reports
pub_type: 社论
doi:10.1007/s00299-017-2135-y
更新日期:2017-05-01 00:00:00
abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
abstract::A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050426
更新日期:1998-04-01 00:00:00
abstract:: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900168
更新日期:2000-06-01 00:00:00
abstract::An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1205-9
更新日期:2012-05-01 00:00:00
abstract:KEY MESSAGE:Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02409-x
更新日期:2019-07-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
abstract:KEY MESSAGE:SbNAC genes (131) encoding 183 proteins were identified from the sorghum genome and characterized. The expression patterns of SbSNACs were evaluated at three sampling time points under post-flowering drought stress. NAC proteins are specific transcription factors in plants, playing vital roles in developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02371-8
更新日期:2019-03-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690259
更新日期:1995-01-01 00:00:00
abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050303
更新日期:1997-07-01 00:00:00
abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1124-9
更新日期:2011-12-01 00:00:00