Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera.

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...

journal_title：Plant cell reports

authors： Wang C,Wang C,Zou J,Yang Y,Li Z,Zhu S

更新日期：2019-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...

journal_title：Plant cell reports

authors： Qin Y,Duan Z,Xia X,Yin W

更新日期：2011-10-01 00:00:00

abstract：:Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...

journal_title：Plant cell reports

authors： Tsuwamoto R,Harada T

更新日期：2011-09-01 00:00:00

abstract：:Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...

journal_title：Plant cell reports

authors： Gilmour DM,Davey MR,Cocking EC

更新日期：1989-05-01 00:00:00

abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

journal_title：Plant cell reports

authors： Nussbaumer P,Kapétanidis I,Christen P

更新日期：1998-03-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

journal_title：Plant cell reports

authors： Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

更新日期：2012-06-01 00:00:00

abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...

journal_title：Plant cell reports

authors： Gao J,Lee JM,An G

更新日期：1991-12-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00

abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

journal_title：Plant cell reports

authors： He DG,Yang YM,Scott KJ

更新日期：1992-02-01 00:00:00

abstract：KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

journal_title：Plant cell reports

authors： Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

更新日期：2018-11-01 00:00:00

abstract：:The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...

journal_title：Plant cell reports

authors： Decendit A,Petit G,Andreu F,Doireau P,Mérillon JM,Rideau M

更新日期：1993-10-01 00:00:00

abstract：:Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...

journal_title：Plant cell reports

authors： Wang F,Wei H,Tong Z,Zhang X,Yang Z,Lan T,Duan Y,Wu W

更新日期：2011-11-01 00:00:00

abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

journal_title：Plant cell reports

authors： Hosoyama H,Irie K,Abe K,Arai S

更新日期：1995-12-01 00:00:00

abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

journal_title：Plant cell reports

authors： Evans DA,Gamborg OL

更新日期：1982-04-01 00:00:00

abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

journal_title：Plant cell reports

authors： Edwards ES,Roux SJ

更新日期：1998-06-01 00:00:00

abstract：:The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...

journal_title：Plant cell reports

authors： Kulpa JM,Galsky AG,Lipetz P,Stephens R

更新日期：1985-04-01 00:00:00

abstract：KEY MESSAGE:We isolated differentially expressed and dark-responsive genes during flower development and opening in petals of morning glory. Flower opening usually depends on petal expansion and is regulated by both genetic and environmental factors. Flower opening in morning glory (Ipomoea nil) is controlled by the da...

journal_title：Plant cell reports

authors： Shinozaki Y,Tanaka R,Ono H,Ogiwara I,Kanekatsu M,van Doorn WG,Yamada T

更新日期：2014-07-01 00:00:00

abstract：:In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...

journal_title：Plant cell reports

authors： Bergmann BA,Moon HK

更新日期：1997-02-01 00:00:00

abstract：:Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

journal_title：Plant cell reports

authors： Padhya MA,Mehta AR

更新日期：1982-12-01 00:00:00

abstract：:Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...

journal_title：Plant cell reports

authors： Nayak P,Sen SK

更新日期：1989-05-01 00:00:00

abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...

journal_title：Plant cell reports

authors： Soares EL,Lima MLB,Nascimento JRS,Soares AA,Coutinho ÍAC,Campos FAP

更新日期：2017-11-01 00:00:00

abstract：:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

journal_title：Plant cell reports

authors： Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

更新日期：1996-01-01 00:00:00

abstract：:Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus t...

journal_title：Plant cell reports

authors： Heyenga AG,Lucas JA,Dewick PM

更新日期：1990-11-01 00:00:00

abstract：:The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...

journal_title：Plant cell reports

authors： Tomaszewska P,Kosina R

更新日期：2019-01-01 00:00:00

abstract：KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

journal_title：Plant cell reports

authors： Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

更新日期：2016-06-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

journal_title：Plant cell reports

authors： Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

更新日期：1998-04-01 00:00:00

abstract：:Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...

journal_title：Plant cell reports

authors： Selby C,McRoberts WC,Hamilton JT,Harvey BM

更新日期：1996-12-01 00:00:00