Abstract：
KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of prolamin species and is the resistant to digestive juices in the intestinal tract. We propose the utilization of PB-Is as an oral vaccine carrier to induce mucosal immune response effectively. If vaccine antigens are localized in a specific layer within PB-Is, they could be protected from gastric juice and be delivered intact to the small intestine. We observed the localization of GFP fluorescence in transgenic rice endosperm expressing prolamin-GFP fusion proteins with native prolamin promoters, and we confirmed that the foreign proteins were located in specific layers of PB-Is artificially. Each prolamin-GFP fusion protein was localized in specific layers of PB-Is, such as the outer-most layer, middle layer, and core region. Furthermore, to investigate the resistance of prolamin-GFP fusion proteins against pepsin digestion, we performed in vitro pepsin treatment. Prolamin-GFP fusion proteins were gradually digested from the peripheral region and the contours of PB-Is were made rough by in vitro pepsin treatment. These findings suggested that prolamin-GFP fusion proteins accumulating specific layers of PB-Is were gradually digested and exposed from the outside by pepsin digestion.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura Tdoi
10.1007/s00299-016-1960-8subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1287-95issue
6eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-016-1960-8journal_volume
35pub_type
杂志文章abstract：KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...
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journal_title：Plant cell reports
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journal_title：Plant cell reports
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pub_type： 杂志文章
doi：10.1007/s00299-004-0910-z
更新日期：2005-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269335
更新日期：1983-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-016-2088-6
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232092
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journal_title：Plant cell reports
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doi：10.1007/s00299-002-0530-4
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pub_type： 杂志文章
doi：10.1007/s002990050576
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doi：10.1007/s00299-011-1016-z
更新日期：2011-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
abstract：:Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-016-2017-8
更新日期：2016-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233425
更新日期：1993-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050436
更新日期：1998-04-01 00:00:00
abstract：:The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232217
更新日期：1996-06-01 00:00:00
abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1591-x
更新日期：2014-05-01 00:00:00
