Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don.

Abstract:

:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average number of stable, geneticin-resistant lines recovered was 0.5 per 200 mg fresh weight bombarded tissue. Expression of the uidA reporter gene was detected histochemically and fluorimetrically in transformed embryogenic tissue and in derived mature somatic embryos and regenerated plants. The integration of uidA and npt II genes into the Pinus radiata genome was demonstrated using PCR amplification of the inserts and Southern hybridisation analysis. The expression of both genes in transformed tissue was confirmed by Northern hybridisation analysis. More than 150 transgenic Pinus radiata plants were produced from 20 independent transformation experiments with four different embryogenic clones.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

doi

10.1007/s002990050426

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

460-468

issue

6-7

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050426

journal_volume

17

pub_type

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